Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Department of Cardiovascular, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5627-5632. doi: 10.3892/mmr.2017.7254. Epub 2017 Aug 16.
Ginkgolide B is one of the components extracted from Folium Ginkgo. It is a natural antagonist of platelet‑activating factor receptor and has multiple pharmacological applications. In the present study, the effect of ginkgolide B on the proliferation of endothelial progenitor cells (EPCs) was examined, as well as its potential underlying mechanism. EPCs were cultured in various concentrations of ginkgolide B (0, 1.25, 5, 20, 80 or 160 µg/ml) for 24 or 48 h and then numbers of viable cells, apoptosis rate and caspace‑3 activity were measured. The results demonstrated that ginkgolide B treatment effectively promoted EPC growth, and suppressed cell apoptosis and caspase‑3 activity compared with control cells. In addition, ginkgolide B treatment significantly induced the expression of miR‑126, vascular endothelial growth factor and endothelial nitric oxide synthase, while it increased phosphorylation of AKT serine/threonine kinase 1 (Akt) and of p38 mitogen‑activated protein kinase in EPCs. The present study therefore demonstrated that ginkgolide B promoted cell growth in EPCs through overexpression of miR‑126 and activation of the Akt signaling pathway.
银杏内酯 B 是从银杏叶中提取的成分之一，是血小板激活因子受体的天然拮抗剂，具有多种药理学作用。本研究探讨了银杏内酯 B 对内皮祖细胞（EPC）增殖的影响及其潜在的作用机制。将 EPC 在不同浓度的银杏内酯 B（0、1.25、5、20、80 或 160μg/ml）中培养 24 或 48h，然后测量活细胞数量、细胞凋亡率和半胱天冬酶-3（caspase-3）活性。结果表明，与对照组细胞相比，银杏内酯 B 处理可有效促进 EPC 生长，并抑制细胞凋亡和 caspase-3 活性。此外，银杏内酯 B 处理可显著诱导 miR-126、血管内皮生长因子和内皮型一氧化氮合酶的表达，同时增加 EPC 中丝氨酸/苏氨酸激酶 1（Akt）和 p38 丝裂原活化蛋白激酶的磷酸化。因此，本研究表明，银杏内酯 B 通过上调 miR-126 和激活 Akt 信号通路促进 EPC 细胞生长。
