Tanigawa Masahiko, Nakayama Masamichi, Taira Tomoki, Hattori Satoshi, Mihara Yutaro, Kondo Reiichiro, Kusano Hironori, Nakamura Ken, Abe Yushi, Ishida Yusuke, Okabe Yoshinobu, Hisaka Toru, Okuda Koji, Fujino Kosuke, Ito Takaaki, Kawahara Akihiko, Naito Yoshiki, Yamaguchi Rin, Akiba Jun, Akagi Yoshito, Yano Hirohisa
Department of Pathology, Kurume University School of Medicine, 67 Asahimachi, Kurume, 830-0011, Japan.
Department of Surgery, Kurume University School of Medicine, Kurume, Japan.
Med Mol Morphol. 2018 Mar;51(1):32-40. doi: 10.1007/s00795-017-0167-6. Epub 2017 Aug 28.
Insulinoma-associated protein 1 (INSM1) is an important biomarker of Achaete-scute homolog-like 1-driven pathways. For diagnosis of pancreatic neuroendocrine tumors (PanNET), chromogranin A (CGA), synaptophysin (SYP), and neural cell adhesion molecule (NCAM) were also considered as potential biomarkers. However, it is often difficult to diagnose it immunohistochemically. Hence, we examined the expression pattern of INSM1 in pancreatic solid tumors. We detected INSM1, CGA, SYP, and NCAM immunohistochemically, in 27 cases of NET [pure type: 25 cases, mixed adenoneuroendocrine carcinoma (MANEC): 2 cases]. We included 5 cases of solid-pseudopapillary neoplasm (SPN), 7 cases of acinar cell carcinoma (ACC), and 15 cases of pancreatic ductal adenocarcinoma (PDAC) as the control group. Nuclear expression of INSM1 was found in all PanNET pure type cases. However, expression of INSM1 was negative in PDAC, ACC, and SPN in all cases, whereas faint expression was seen in the cytoplasm from SPN. MANEC comprises of two components: neuroendocrine carcinoma and adenocarcinoma components. The NET component was positive for INSM1 expression, whereas the PDAC component does not express INSM1, which aids in distinguishing these components. Our results suggest that INSM1 is a useful immunohistochemical marker for diagnosing pancreatic neuroendocrine tumor.
胰岛素瘤相关蛋白1（INSM1）是achaete - scute同源样蛋白1驱动通路的重要生物标志物。对于胰腺神经内分泌肿瘤（PanNET）的诊断，嗜铬粒蛋白A（CGA）、突触素（SYP）和神经细胞黏附分子（NCAM）也被视为潜在的生物标志物。然而，通过免疫组织化学方法诊断往往存在困难。因此，我们研究了INSM1在胰腺实性肿瘤中的表达模式。我们采用免疫组织化学方法检测了27例神经内分泌肿瘤（NET）[纯型：25例，混合性腺神经内分泌癌（MANEC）：2例]中的INSM1、CGA、SYP和NCAM。我们纳入了5例实性假乳头状肿瘤（SPN）、7例腺泡细胞癌（ACC）和15例胰腺导管腺癌（PDAC）作为对照组。在所有PanNET纯型病例中均发现INSM1的核表达。然而，在所有病例中，PDAC、ACC和SPN中INSM1的表达均为阴性，而SPN的细胞质中有微弱表达。MANEC由两种成分组成：神经内分泌癌和腺癌成分。NET成分中INSM1表达阳性，而PDAC成分不表达INSM1，这有助于区分这些成分。我们的结果表明，INSM1是诊断胰腺神经内分泌肿瘤的一种有用的免疫组织化学标志物。
