Department of Nursing, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
Department of Occupational and Environmental Health, Dalian Medical University, Dalian, Liaoning, China.
Adv Exp Med Biol. 2017;975 Pt 2:917-927. doi: 10.1007/978-94-024-1079-2_73.
The purpose of this study was to explore the protective capacity of taurine on arsenic (As)-induced neurotoxicity. Thirty mice were used and ten rats in each group. We treated the As exposure group with 4 ppm AsO for 60 days by drinking water and the protective group with 4 ppm AsO and 150 mg/kg taurine. Drinking water was only given in the control group. Pathologic changes and DNA damage in the mice kidney were examined by HE staining, immunohistochemistry and comet assay. Abnormal morphological changes were found in the kidney of As exposed mouse. Moreover, 8-hydroxy-2-deoxyguanosine (8-OHdG) expression and comet number, tail moment, and tail length of comet were markedly elevated in the As intoxication mice. However, histopathological changes and low expression of 8-OHdG were shown in the protective group. Our results indicate that supplementation of taurine protects against the histopathologic changes and DNA damage of mouse kidneys in As exposure group.
本研究旨在探讨牛磺酸对砷（As）诱导的神经毒性的保护作用。将 30 只小鼠分为 10 组，每组 10 只大鼠。我们通过饮用水给暴露于 As 的组给予 4ppmAsO 处理 60 天，而保护组则给予 4ppmAsO 和 150mg/kg 牛磺酸。仅在对照组给予饮用水。通过 HE 染色、免疫组织化学和彗星试验检查小鼠肾脏的病理变化和 DNA 损伤。在暴露于 As 的小鼠肾脏中发现了异常的形态变化。此外，在 As 中毒小鼠中，8-羟基-2-脱氧鸟苷（8-OHdG）表达和彗星的数量、尾部矩和尾部长度明显升高。然而，在保护组中显示出组织病理学变化和 8-OHdG 的低表达。我们的结果表明，牛磺酸的补充可防止暴露于 As 组小鼠肾脏的组织病理学变化和 DNA 损伤。
