Department of Biotechnology, Konkuk University, Chungju, 27478, South Korea.
Korea Nokyong Research Center, Konkuk University, Chungju, 27478, South Korea.
Adv Exp Med Biol. 2017;975 Pt 2:1141-1152. doi: 10.1007/978-94-024-1079-2_91.
This prime objective of this study was to explore the anti-cancer activity of fermented Asterina pectinifera with Cordyceps militaris mycelia (FACM) in B16F10 murine melanoma cells. The effect of FACM on cell viability was assessed using MTT assay. Furthermore, the effect of FACM was compared with unfermented A. pectinifera on cell viability. The results demonstrated that the fermented FACM extract has a higher inhibitory activity on the proliferation of B16F10 murine melanoma cells than unfermented A. pectinifera. In addition, FACM also promoted the expression of pro-apoptotic protein Bax leading to stimulate apoptosis in B16F10 cells. Therefore the present study demonstrates that the FACM might be a potential effective anti-cancer agent, as a result of its stronger anti-proliferative effect and apoptosis inducing effect than A. pectinifera or C. militaris on melanoma cells.
本研究的主要目的是探索发酵后的海星和蛹虫草菌丝体（FACM）对 B16F10 鼠黑色素瘤细胞的抗癌活性。采用 MTT 法评估 FACM 对细胞活力的影响。此外，还比较了发酵前后的海星对细胞活力的影响。结果表明，发酵后的 FACM 提取物对 B16F10 鼠黑色素瘤细胞的增殖具有更高的抑制活性，比未发酵的海星更强。此外，FACM 还促进了促凋亡蛋白 Bax 的表达，从而刺激 B16F10 细胞凋亡。因此，本研究表明，与海星或蛹虫草相比，FACM 可能是一种潜在有效的抗癌药物，因为它对黑色素瘤细胞具有更强的抗增殖作用和诱导凋亡作用。
