Key Laboratory of Food Bio-technology, School of Food and Bioengineering, Xihua University, Chengdu 610039, PR China.
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2019 Jun 1;130:307-314. doi: 10.1016/j.ijbiomac.2019.02.155. Epub 2019 Feb 27.
A comparison of the anti-tumor activity of CMPS-II and CBPS-II polysaccharides, respectively is obtained from the fermented mycelium and cultivated fruiting bodies of Cordyceps militaris. This in vitro anti-tumor activity is investigated using an MTT assay, immunofluorescence staining, a Western Blot assay, a qRT-PCR assay, and Annexin V-FITC/PI double staining. The experimental results indicate that the inhibition rate of CMPS-II on H1299 tumor cells is higher than that of CBPS-II. With a concentration of 500 μ g/mL, the inhibition rate of CMPS-II and CBPS-II were 54.55% and 34.80%, respectively. Both CMPS-II and CBPS-II can increase the protein and mRNA expression level of cell apoptosis factors Caspase-3, Caspase-9, and p53, while reducing the protein and mRNA expression levels of proliferating cell nuclear antigen (PCNA), to induce tumor cells apoptosis. The induction effect of CMPS-II was stronger than CBPS-II. These results suggest that CMPS-II is superior to CBPS-II regarding the inhibition of H1299 lung cancer cells. Furthermore, CMPS-II is a potentially useful substitution for CBPS-II in the treatment of lung cancer and provides new insights into the mechanism of its anti-tumor activity.
从蛹虫草发酵菌丝体和培养子实体中分别获得 CMPS-II 和 CBPS-II 多糖的抗肿瘤活性比较。采用 MTT 法、免疫荧光染色法、Western blot 法、qRT-PCR 法和 Annexin V-FITC/PI 双染法对其体外抗肿瘤活性进行了研究。实验结果表明，CMPS-II 对 H1299 肿瘤细胞的抑制率高于 CBPS-II。在 500μg/mL 浓度下，CMPS-II 和 CBPS-II 的抑制率分别为 54.55%和 34.80%。CMPS-II 和 CBPS-II 均可增加细胞凋亡因子 Caspase-3、Caspase-9 和 p53 的蛋白和 mRNA 表达水平，同时降低增殖细胞核抗原（PCNA）的蛋白和 mRNA 表达水平，诱导肿瘤细胞凋亡。CMPS-II 的诱导作用强于 CBPS-II。这些结果表明，CMPS-II 在抑制 H1299 肺癌细胞方面优于 CBPS-II。此外，CMPS-II 可能是治疗肺癌的 CBPS-II 的替代品，为其抗肿瘤活性的机制提供了新的见解。
