Department of Biotechnology, Konkuk University, Chungju, 27478, South Korea.
Korea Nokyong Research Center, Konkuk University, Chungju, 27478, South Korea.
Adv Exp Med Biol. 2017;975 Pt 2:1179-1190. doi: 10.1007/978-94-024-1079-2_94.
This study aimed to investigate the effect of doxorubicin co-treatment with taurine on B16F10 melanoma cells. Frequently, Doxorubicin is used in the treatments of many different kinds of cancers, some of which are soft tissue sarcomas, hematological malignancies and carcinomas. However, the clinical application of doxorubicin is compromised by its severe adverse effects, including cardiotoxicity. In the present study, the efficacy of doxorubicin co-treatment with taurine was investigated. B16F10 cell viability was evaluated using MTT assays, trypan blue dye exclusion assays, and fluorescent staining technique. Apoptotic cells were detected by flow cytometry and the proteins associated with apoptosis and cellular differentiations were assessed by immunoblotting. Doxorubicin inhibited cell growth and induced cell death in B16F10 cells. Interestingly, doxorubicin co-treatment with taurine inhibited apoptosis in B16F10 cells. These results indicate that doxorubicin co-treatment with taurine attenuates doxorubicin-induced cytotoxicity and reduces ROS production in B16F10 cells.
本研究旨在探讨牛磺酸与阿霉素联合处理对 B16F10 黑素瘤细胞的影响。阿霉素常用于治疗多种不同类型的癌症，包括软组织肉瘤、血液恶性肿瘤和癌。然而，阿霉素的临床应用受到其严重不良反应的限制，包括心脏毒性。在本研究中，研究了牛磺酸与阿霉素联合处理的疗效。通过 MTT 分析、台盼蓝排斥试验和荧光染色技术评估 B16F10 细胞活力。通过流式细胞术检测凋亡细胞，并通过免疫印迹评估与细胞凋亡和细胞分化相关的蛋白质。阿霉素抑制 B16F10 细胞的生长并诱导细胞死亡。有趣的是，阿霉素与牛磺酸联合处理抑制了 B16F10 细胞的凋亡。这些结果表明，牛磺酸与阿霉素联合处理可减轻阿霉素诱导的细胞毒性并减少 B16F10 细胞中 ROS 的产生。
