Division of Food Bioscience, Konkuk University, Chungju, South Korea.
Division Korea Nokyong Research Center, Konkuk University, Chungju, South Korea.
Adv Exp Med Biol. 2017;975 Pt 2:1191-1201. doi: 10.1007/978-94-024-1079-2_95.
Nephrotic syndrome is still a therapeutic challenge because an effective treatment has not been developed. Evidence suggests that multidrug therapy is more effective than monotherapy in amelioration of renal injury. Therefore, we examined if taurine exerts a protective effect on doxorubicin-induced acute kidney injury in mice. Eight-week-old male Balb/c nude mice were used in this study. Taurine was orally administered at a dose of 50 mg/kg and 100 mg/kg body weight for 5 days. In the meantime, the mice were administered intraperitoneal injections of doxorubicin at 15 mg/kg body weight. At 24 h after the doxorubicin challenge, the response in the taurine-treated mice was compared with that in the vehicle-treated control mice. The doxorubicin-induced acute kidney injury model displayed a significant increase in the renal expression of apoptosis-related proteins (p53, phospho-p53, caspase 9, and caspase 3), whereas in the taurine-treated mice, the augmented expression of renal inflammation-related mRNAs such as NF-kB, COX-2, and iNOS was down-regulated. These results suggest that taurine acts as a renoprotective agent by inhibiting apoptosis and inflammation in the kidney of mice with doxorubicin-induced renal injury.
肾病综合征仍然是一个治疗挑战，因为尚未开发出有效的治疗方法。有证据表明，多药物治疗比单药治疗更能改善肾脏损伤。因此，我们研究了牛磺酸是否对阿霉素诱导的小鼠急性肾损伤有保护作用。本研究使用 8 周龄雄性 Balb/c 裸鼠。牛磺酸以 50mg/kg 和 100mg/kg 体重的剂量口服给药 5 天。同时，给小鼠腹腔注射 15mg/kg 体重的阿霉素。在阿霉素攻击后 24 小时，将牛磺酸处理组小鼠的反应与载体处理的对照组小鼠进行比较。阿霉素诱导的急性肾损伤模型显示，凋亡相关蛋白（p53、磷酸化 p53、caspase 9 和 caspase 3）在肾脏中的表达显著增加，而在牛磺酸处理的小鼠中，肾脏炎症相关 mRNA（如 NF-kB、COX-2 和 iNOS）的增强表达被下调。这些结果表明，牛磺酸通过抑制阿霉素诱导的肾损伤小鼠肾脏中的细胞凋亡和炎症发挥肾保护作用。
