钙和钙调蛋白在调节血小板鸟苷酸环化酶活性中的作用研究。
Investigation of the role of Ca2+ and calmodulin in the regulation of platelet guanylate cyclase activity.
作者信息
Mac Neil S
出版信息
Biochem J. 1987 Mar 1;242(2):607-10. doi: 10.1042/bj2420607.
Abstract
Both soluble and particulate forms of human platelet guanylate cyclase were found to be sensitive to sub-micromolar concentrations of free Ca2+; soluble enzyme activity increased as Ca2+ was increased from 10 nM to 1 microM; particulate enzyme activity showed a biphasic response to Ca2+, with maximal enzyme activity between 1 and 10 nM free Ca2+ and inhibition occurring at higher Ca2+ concentrations. Neither Ca2+-sensitivity appeared to be calmodulin-dependent.
摘要
已发现人血小板鸟苷酸环化酶的可溶性和颗粒性形式均对亚微摩尔浓度的游离Ca2+敏感；随着Ca2+浓度从10 nM增加到1 μM，可溶性酶活性增强；颗粒性酶活性对Ca2+呈现双相反应，在游离Ca2+浓度为1至10 nM时酶活性最高，而在较高Ca2+浓度时出现抑制。两种Ca2+敏感性似乎均不依赖钙调蛋白。
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