Bell A J, Figes A, Oscier D G, Hamblin T J
Br J Haematol. 1987 May;66(1):63-8. doi: 10.1111/j.1365-2141.1987.tb06891.x.
Large numbers of circulating haemopoietic progenitor cells were collected from three patients with lymphoid neoplasms for the purpose of haemopoietic reconstitution following intensive chemotherapy or chemoradiotherapy. Each patient underwent four to six leukaphereses at a time when the circulating stem cell pool was expanded. 2-3 weeks after the end of myelosuppressive chemotherapy. The peripheral blood mononuclear cells (PBMNC) thus obtained were assayed for granulocyte-macrophage colony-forming cells (CFU-GM) and were cryopreserved in liquid nitrogen. Two patients were autografted with PBMNC containing 60 X 10(4) and 76 X 10(4) CFU-GM/kg body weight respectively. Both these patients showed prompt engraftment which is stable at +10 and +6 months. A third patient who was autografted with PBMNC containing 33 X 10(4) CFU-GM showed only temporary and incomplete engraftment. These observations confirm that the peripheral blood may be used as a source of haemopoietic stem cells for autografting, but criteria for predicting engraftment are at present uncertain.
为了在强化化疗或放化疗后进行造血重建，从三名淋巴瘤患者体内采集了大量循环造血祖细胞。每位患者在循环干细胞池扩张时进行了四到六次白细胞单采术，即在骨髓抑制性化疗结束后2至3周。对由此获得的外周血单个核细胞（PBMNC）进行粒细胞-巨噬细胞集落形成细胞（CFU-GM）检测，并液氮冻存。两名患者分别接受了含有60×10⁴和76×10⁴CFU-GM/kg体重的PBMNC自体移植。这两名患者均迅速植入，在+10个月和+6个月时情况稳定。第三名患者接受了含有33×10⁴CFU-GM的PBMNC自体移植，仅表现出暂时和不完全的植入。这些观察结果证实外周血可作为自体移植的造血干细胞来源，但目前预测植入的标准尚不确定。
