Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, 1205, Bangladesh.
BMC Microbiol. 2017 Aug 29;17(1):189. doi: 10.1186/s12866-017-1099-y.
Improved methods with better separation and concentration ability for detection of foodborne pathogens are in constant need. The aim of this study was to evaluate microplate immunocapture (IC) method for detection of Salmonella Typhi, Shigella flexneri and Vibrio cholerae from food samples to provide a better alternative to conventional culture based methods.
The IC method was optimized for incubation time, bacterial concentration, and capture efficiency. 6 h incubation and log 6 CFU/ml cell concentration provided optimal results. The method was shown to be highly specific for the pathogens concerned. Capture efficiency (CE) was around 100% of the target pathogens, whereas CE was either zero or very low for non-target pathogens. The IC method also showed better pathogen detection ability at different concentrations of cells from artificially contaminated food samples in comparison with culture based methods. Performance parameter of the method was also comparable (Detection limit- 25 CFU/25 g; sensitivity 100%; specificity-96.8%; Accuracy-96.7%), even better than culture based methods (Detection limit- 125 CFU/25 g; sensitivity 95.9%; specificity-97%; Accuracy-96.2%).
The IC method poses to be the potential to be used as a method of choice for detection of foodborne pathogens in routine laboratory practice after proper validation.
需要改进具有更好分离和浓缩能力的方法来检测食源性致病菌。本研究旨在评估微板免疫捕获（IC）方法用于检测食品中的伤寒沙门氏菌、福氏志贺菌和霍乱弧菌，为传统基于培养的方法提供更好的替代方法。
优化了 IC 方法的孵育时间、细菌浓度和捕获效率。6 小时孵育和 log6CFU/ml 细胞浓度提供了最佳结果。该方法对相关病原体具有高度特异性。目标病原体的捕获效率（CE）约为 100%，而非目标病原体的 CE 要么为零，要么非常低。与基于培养的方法相比，IC 方法在人工污染食品样品中不同细胞浓度下也显示出更好的病原体检测能力。该方法的性能参数也相当（检测限-25 CFU/25 g；灵敏度 100%；特异性-96.8%；准确性-96.7%），甚至优于基于培养的方法（检测限-125 CFU/25 g；灵敏度 95.9%；特异性-97%；准确性-96.2%）。
在适当验证后，IC 方法有望成为常规实验室实践中检测食源性致病菌的首选方法。
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