Zhao Xihong, Lin Chii-Wann, Wang Jun, Oh Deog Hwan
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China, Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200701, Republic of Korea, Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
J Microbiol Biotechnol. 2014 Mar 28;24(3):297-312. doi: 10.4014/jmb.1310.10013.
Food safety is increasingly becoming an important public health issue, as foodborne diseases present a widespread and growing public health problem in both developed and developing countries. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent human foodborne infections. Traditional microbiological detection and identification methods for foodborne pathogens are well known to be time consuming and laborious as they are increasingly being perceived as insufficient to meet the demands of rapid food testing. Recently, various kinds of rapid detection, identification, and monitoring methods have been developed for foodborne pathogens, including nucleic-acid-based methods, immunological methods, and biosensor-based methods, etc. This article reviews the principles, characteristics, and applications of recent rapid detection methods for foodborne pathogens.
食品安全日益成为一个重要的公共卫生问题，因为食源性疾病在发达国家和发展中国家都是一个普遍且日益严重的公共卫生问题。食源性病原体的快速精准监测和检测是控制和预防人类食源性感染的一些最有效方法。众所周知，传统的食源性病原体微生物检测和鉴定方法既耗时又费力，因为人们越来越认为它们不足以满足快速食品检测的需求。近年来，已开发出各种用于食源性病原体的快速检测、鉴定和监测方法，包括基于核酸的方法、免疫方法和基于生物传感器的方法等。本文综述了近年来食源性病原体快速检测方法的原理、特点及应用。
