Schmid College of Science and Technology, Food Science Program, Chapman University, One University Drive, Orange, CA 92866, USA.
Schmid College of Science and Technology, Food Science Program, Chapman University, One University Drive, Orange, CA 92866, USA.
J Microbiol Methods. 2020 May;172:105881. doi: 10.1016/j.mimet.2020.105881. Epub 2020 Feb 28.
The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of Salmonella in pet food with the 3 M Molecular Detection System (MDS) or selective plating on XLD. Dog food and pig ear treats were inoculated with Salmonella Infantis at concentrations of 10-10 CFU/25 g, followed by a 3-h enrichment, then microplate IC and 3 M MDS or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by 3 M MDS or selective plating. Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of Salmonella in 100% of dog food and treat samples tested, including at levels as low as 10 CFU/25 g. Microplate IC coupled with 3 M MDS enabled detection of Salmonella in dog food and treat samples down to levels of 10 CFU/25 g, with an overall detection rate of 92%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of Salmonella in complex matrices when coupled with 3 M MDS or selective plating.
本研究的目的是使用微孔板免疫捕获（IC）来减少使用 3M 分子检测系统（MDS）或 XLD 选择性平板检测宠物食品中沙门氏菌所需的富集时间。将婴儿沙门氏菌以 10-10 CFU/25 g 的浓度接种于狗食和猪耳零食中，然后进行 3 小时的富集，随后进行微孔板 IC 和 3M MDS 或微孔板 IC 和 XLD 选择性平板检测。另一组样品进行传统的 24 小时富集，然后进行 3M MDS 或选择性平板检测。基于三个独立试验的结果，微孔板 IC 随后进行选择性平板检测能够检测到 100%的狗食和零食样本中的沙门氏菌，包括低至 10 CFU/25 g 的水平。微孔板 IC 与 3M MDS 结合使用能够检测到狗食和零食样本中的沙门氏菌，低至 10 CFU/25 g，总检测率为 92%。这些结果表明，微孔板 IC 具有很高的潜力，可以替代传统的 24 小时富集步骤，与 3M MDS 或选择性平板检测结合使用时，能够检测复杂基质中的沙门氏菌。
