College of Life Science, Shandong Agricultural University, Taian, 271018, PR China.
Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China.
Int J Biol Macromol. 2018 Jan;106:1270-1278. doi: 10.1016/j.ijbiomac.2017.08.138. Epub 2017 Aug 26.
Two extractable polysaccharides of Ac-MPS and Al-MPS were extracted from the mycelium of Agrocybe aegerita (Brig.) Sing and the in vitro antioxidant activities and in vivo anti-aging activities in a d-gal-induced aging mice were investigated in the present study. The in vitro antioxidant analysis showed that Ac-MPS showed superior scavenging effects on hydroxyl of 68.62±2.59% and DPPH of 85.19±3.26% at the concentration of 4500μg/mL. Biochemical indices and antioxidant analysis in vivo showed that Ac-MPS had better effects in raising hepatic activities of SOD, CAT, GPx and T-AOC, inhibiting hepatic contents of LPO and MDA, as well as improving serum lipid levels of LDL-C, HDL-C, LDL-C/HDL-C, TG and TC. Furthermore, HYP measurements suggested that skin collagen in aging mice could be maintained. This study suggested that both Ac-MPS and Al-MPS could be used as potential natural agents or dietary supplements in attenuating aging and its complications.
从阿魏侧耳（Brig.）Sing 的菌丝体中提取了两种可提取的多糖 Ac-MPS 和 Al-MPS，并研究了它们在体外对羟基的清除作用和体内对 D-半乳糖诱导的衰老小鼠的抗衰老作用。体外抗氧化分析表明，Ac-MPS 在 4500μg/mL 浓度下对羟基的清除效果为 68.62±2.59%，对 DPPH 的清除效果为 85.19±3.26%。体内生化指标和抗氧化分析表明，Ac-MPS 能更有效地提高肝组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和总抗氧化能力（T-AOC）的活性，抑制肝组织中丙二醛（MDA）和脂质过氧化物（LPO）的含量，改善血清中 LDL-C、HDL-C、LDL-C/HDL-C、甘油三酯（TG）和总胆固醇（TC）的水平。此外，羟脯氨酸（HYP）测量表明，衰老小鼠皮肤的胶原蛋白可以得到维持。本研究表明，Ac-MPS 和 Al-MPS 均可作为潜在的天然药物或膳食补充剂，用于减轻衰老及其并发症。
