Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling (FABI), Center for Pharmaceutical Research (CePhaR), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium; Laboratory of Chemical, Galenic and Pharmacological Development of Drugs, Faculty of Pharmacy, Monastir, Tunisia.
Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling (FABI), Center for Pharmaceutical Research (CePhaR), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
Int J Biol Macromol. 2018 Jan;106:1071-1079. doi: 10.1016/j.ijbiomac.2017.08.107. Epub 2017 Aug 26.
The aim of this study is to determine the phytochemical profile, the total polyphenolic contents and the antioxidant activities of Haplophyllum tuberculatum leaves extracts. The most active extracts were analyzed by ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry. Antioxidant activities were screened by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) test and measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and β-carotene bleaching inhibition assays. Phytochemical screening of the extracts revealed the presence of various secondary metabolites. The ethyl acetate extract was the richest extract in phenolics and flavonoids with 262mg gallic acid equivalents/g and 99.1mg quercetin equivalent/g of dry weight, respectively. The same extract showed an important scavenging effect on DPPH, ABTS and β-carotene/linoleic acid with IC of 0.020mg/mL, 0.029mg/mL and 0.022mg/mL, respectively. The correlations between the antioxidant capacities and the polyphenolic content were ranging between 0.889 and 0.256 and occasionally found to be significant. The UPLC-ESI-MS analysis showed the presence of polyphenolic and alkaloid compounds. Arabelline, majidine, dictamine and a qudsine derivative are found for the first time in H. tuberculatum. The results indicate that polyphenolic and alkaloid compounds may be major contributors to the antioxidant activity of these extracts.
本研究旨在确定糙叶树 Haplophyllum tuberculatum 叶片提取物的植物化学成分谱、总多酚含量和抗氧化活性。通过超高效液相色谱-电喷雾电离质谱联用对最具活性的提取物进行分析。采用 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）试验筛选抗氧化活性，并通过 2,2-二苯基-1-苦基肼基（DPPH）、2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和β-胡萝卜素漂白抑制试验来测量抗氧化活性。提取物的植物化学成分筛选表明存在各种次生代谢物。乙酸乙酯提取物是酚类和类黄酮含量最丰富的提取物，分别为 262mg 没食子酸当量/g 和 99.1mg 槲皮素当量/g 干重。相同的提取物对 DPPH、ABTS 和 β-胡萝卜素/亚油酸具有重要的清除作用，IC 分别为 0.020mg/mL、0.029mg/mL 和 0.022mg/mL。抗氧化能力与多酚含量之间的相关性在 0.889 到 0.256 之间，有时发现具有显著相关性。UPLC-ESI-MS 分析表明存在多酚和生物碱化合物。在糙叶树中首次发现了阿拉伯灵碱、马吉定、地丁宁和一种喹丁衍生物。结果表明，多酚和生物碱化合物可能是这些提取物抗氧化活性的主要贡献者。
