Priyadarshi Himanshu, Das Rekha, Pavan-Kumar Annam, Gireesh-Babu Pathakota, Javed Hasan, Kumar Sujit, Marappan Makesh, Krishna Gopal, Chaudhari Aparna
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India.
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
J Exp Biol. 2017 Nov 15;220(Pt 22):4101-4108. doi: 10.1242/jeb.163410. Epub 2017 Aug 29.
Morphotypic differentiation is the external manifestation of dominance hierarchy in The intermediate morphotype orange claw (OC) male exhibits the highest growth rate and is subordinate in hierarchy to blue claw (BC) male while dominant on small male (SM). The present study was undertaken to examine the specific role of insulin-like androgenic gland () hormone in morphotype differentiation of To achieve this, RNAi mediated knockdown as well as augmentation of transcripts were effected in ∼60 g OC males using plasmid-based constructs pcD-IAG-lh and pcD-IAGorf, respectively. The treatments were administered to animals maintained in isolation as well as in community. The knockdown plasmid construct that expresses -specific long hairpin RNA caused 16-fold reduction of transcripts in the SSN1 cell line When injected into OC males living in a community, 2.3-fold knockdown was recorded, while in isolated OC males it was 4.2-fold initially, but returned to normal subsequently. Compared with the respective controls, OC to BC transformations in the silenced animals were significantly lower in the community-reared group, while no difference was observed in the isolated animals. It is reported here for the first time that augmentation in OC males resulted in significantly higher OC to BC transformations, when animals were reared in community. This plasmid-based IAG knockdown approach could be developed into a low stress, feed or immersion treatment for controlling heterogeneous individual growth of males in aquaculture.
形态型分化是十足目动物优势等级制度的外在表现。中间形态型橙色螯（OC）雄蟹生长速度最快，在等级制度中从属于蓝色螯（BC）雄蟹，但对小型雄蟹（SM）具有优势。本研究旨在探讨胰岛素样雄激素腺（IAG）激素在十足目动物形态型分化中的具体作用。为此，分别使用基于质粒的构建体pcD-IAG-lh和pcD-IAGorf，在约60克的OC雄蟹中通过RNA干扰介导敲低以及增加IAG转录本。这些处理应用于单独饲养以及群体饲养的动物。表达IAG特异性长链发夹RNA的敲低质粒构建体导致SSN1细胞系中IAG转录本减少16倍。当注射到群体饲养的OC雄蟹中时，记录到IAG敲低2.3倍，而在单独饲养的OC雄蟹中，最初敲低4.2倍，但随后恢复正常。与各自的对照组相比，在群体饲养组中，IAG沉默动物从OC向BC的转变显著更低，而在单独饲养的动物中未观察到差异。首次在此报道，当动物群体饲养时，OC雄蟹中IAG增加导致从OC向BC的转变显著更高。这种基于质粒的IAG敲低方法可发展成为一种低应激的饲料或浸泡处理方法，用于控制水产养殖中十足目雄蟹个体的异质生长。
