Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology, Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China.
Int J Mol Sci. 2020 Jun 12;21(12):4207. doi: 10.3390/ijms21124207.
Giant freshwater prawns () are commonly found throughout the world. The size of the male giant freshwater prawn is much larger than that of the female. Therefore, understanding the molecular mechanism that underlies the sexual differentiation of is of both commercial and scientific importance. Insulin-like androgenic gland hormone (IAG) plays a key role in the differentiation of sex in . Although IAG has been investigated, the regulatory relationship between IAG and its binding protein partner, the insulin-like androgenic gland hormone-binding protein (IAGBP), has not been studied in . Here, we cloned and characterized the from () for the very first time. Transcriptomic analysis showed that mRNA was detected in a wide array of tissues with the highest expression found in the androgenic gland. The importance of in male development was further demonstrated by an increase in transcripts during the development of the androgenic gland and was only highly transcribed in the androgenic gland of . Interestingly, we found that the gene expression started during the 20th-day larva after hatching stage (LH20), followed (20th-day post-larval stage, PL20) by a gradual elevation of levels. The levels of both genes peaked at the adult stage. The relationship between - and - was further analyzed using RNA interference. The injection of double-stranded RNA (dsRNA) significantly reduced the transcription of , while the amount of mRNA and the translation of IAGBP protein was significantly reduced by the injection of - dsRNA. These results revealed that IAGBP is involved in IAG signaling. Furthermore, our data supports the hypothesis that (IAG and IAGBP)-IAG receptor signaling schemes exist in . Our results will provide important information for the further study of determining the sex of .
淡水巨型螯虾（）在世界范围内广泛分布。雄性淡水巨型螯虾的体型明显大于雌性。因此，了解导致雄性和雌性分化的分子机制在商业和科学上都具有重要意义。胰岛素样雄激素腺激素（IAG）在 （）的性别分化中起着关键作用。尽管已经对 IAG 进行了研究，但在 （）中，IAG 与其结合蛋白伴侣胰岛素样雄激素腺激素结合蛋白（IAGBP）之间的调节关系尚未得到研究。在这里，我们首次从 （）克隆和鉴定了 。转录组分析表明，在广泛的组织中均检测到 mRNA 的表达，其中在雄激素腺中的表达最高。 在雄性发育中的重要性进一步通过雄激素腺发育过程中 转录本的增加得到证明，并且 仅在 的雄激素腺中高度转录。有趣的是，我们发现 基因表达始于孵化后第 20 天幼虫期（LH20），随后（第 20 天幼体后期，PL20） 水平逐渐升高。这两个基因的水平在成虫期达到峰值。 使用 RNA 干扰进一步分析了 - 与 - 的关系。注射双链 RNA（dsRNA）可显著降低 的转录，而注射 - dsRNA 可显著降低 IAGBP 蛋白的 mRNA 量和翻译。这些结果表明 IAGBP 参与了 IAG 信号转导。此外，我们的数据支持了在 （IAG 和 IAGBP）-IAG 受体信号转导方案存在的假设。我们的研究结果将为进一步研究确定 的性别提供重要信息。
