Strauchen J A, Breakstone B
Hematol Oncol. 1987 Apr-Jun;5(2):107-14. doi: 10.1002/hon.2900050205.
Expression of the granulocyte antigen Leu M-1 is characteristic of Reed-Sternberg cells and the related mononuclear cells of Hodgkin's Disease. Leu M-1 has been proposed as a specific immunological marker for Hodgkin's Disease which may be otherwise difficult to distinguish both morphologically and immunologically from non Hodgkin's lymphomas of peripheral T-cell type. In the present study the comparative expression of Leu M-1 in Hodgkin's Disease and peripheral T cell lymphoma was studied in a series of 43 cases including 25 cases of Hodgkin's Disease and 18 cases of immunologically documented peripheral T cell lymphoma. Leu M-1 staining by avidin-biotin-peroxidase complex technique in acetone fixed frozen sections was observed in 22 of 25 cases of Hodgkin's Diseases, (2 of 3 cases of lymphocyte predominant Hodgkin's Disease and 1 case of mixed cellularity were negative) and in 4 of 18 cases of peripheral T cell lymphoma. The pattern of staining in the peripheral T cell lymphomas was indistinguishable from that observed in Hodgkin's Disease in 2 of the cases. Leu M-1 staining appears to be of limited diagnostic value in the differential diagnosis of Hodgkin's Disease and T cell lymphoma. Absence of Leu M-1 staining in frozen tissue however, makes a diagnosis of Hodgkin's Disease (with the exception of the lymphocyte predominant form) unlikely.
粒细胞抗原Leu M-1的表达是里德-斯腾伯格细胞及霍奇金病相关单核细胞的特征。Leu M-1已被提议作为霍奇金病的一种特异性免疫标志物，否则在形态学和免疫学上可能难以将其与外周T细胞型非霍奇金淋巴瘤区分开来。在本研究中，对43例病例进行了研究，包括25例霍奇金病和18例经免疫学证实的外周T细胞淋巴瘤，以比较Leu M-1在霍奇金病和外周T细胞淋巴瘤中的表达情况。在丙酮固定的冰冻切片中，采用抗生物素蛋白-生物素-过氧化物酶复合物技术进行Leu M-1染色，结果显示，25例霍奇金病中有22例呈阳性（3例淋巴细胞为主型霍奇金病中有2例及1例混合细胞型为阴性），18例外周T细胞淋巴瘤中有4例呈阳性。在2例外周T细胞淋巴瘤中，染色模式与霍奇金病中观察到的无法区分。Leu M-1染色在霍奇金病和T细胞淋巴瘤的鉴别诊断中似乎诊断价值有限。然而，冰冻组织中缺乏Leu M-1染色则不太可能诊断为霍奇金病（淋巴细胞为主型除外）。
