氧化应激对雏菊愈伤组织培养中酚类成分和抗氧化代谢的影响

THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY.

作者信息

Karakas Fatma Pehlivan, Cingoz Gunce Sahin, Turker Arzu Ucar

机构信息

Department of Field Crops, Faculty of Agriculture and Natural Sciences, Abant Izzet Baysal University, Bolu, Turkey.

Department of Biology, Faculty of Science and Art, Abant Izzet Baysal University, Bolu, Turkey.

出版信息

Afr J Tradit Complement Altern Med. 2016 Jul 3;13(4):34-41. doi: 10.21010/ajtcam.v13i4.6. eCollection 2016.

DOI:10.21010/ajtcam.v13i4.6

PMID:28852718

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5566151/

Abstract

BACKGROUND

Exogenous pretreatment of oxidative stress on callus cultures of L. (common daisy) induced catalase (CAT), superoxide dismutase (SOD), total phenolic, total flavonoid, total protein and selected commercial phenolic compounds production and accumulation.

MATERIALS AND METHODS

The callus culture obtained from pedicel explants was incubated on Murashige and Skoog medium (MS) containing 10 mM HO or 0 mM HO (for control group) for 10 hours. Twenty phenolic compounds (apigenin, caffeic acid, -coumaric acid, gallic acid, genistein, kaempferol, luteolin, myricetin, procyanidin-, quercetin, rutin hydrate, vanillic acid, ferulic acid, salicylic acid, sinapic acid, chlorogenic acid, hesperedin, naringenin, rosmarinic acid and isorhamnetin) were detected by LC-ESI-MS/MS analysis in methanolic extracts of 10 mM HO and control treatments.

RESULTS

A predominant phenolic compound was chlorogenic acid followed by rutin hydrate, caffeic acid, luteoline, isorhamnetin, quercetin, myricetin, apigenin, -coumaric acid and kaempferol. No gallic acid, genistein, procyanidin-, vanillic acid, sinapic acid, hesperidin and naringenin were detected in HO treatment and control groups of . The total phenolic contents estimated were in the order of HO treatment (285.36 μg/g dw) and control (220.79 μg/g dw) groups. The biosynthesis and accumulation of kaempferol, myricetin, quercetin and isorhamnetin were only determined in HO treatment callus materials. The HO pretreatment clearly showed in a raise in enzymatic and non-enzymatic antioxidant activities. Finally, a significant positive correlation between phenolic accumulation and comprehensive activities of CAT, SOD, total phenolic, total flavonoid and proline was accessible.

CONCLUSION

The present results suggest that using HO as an elicitor or a stimulant plays a significant enhancement role in special phenolic molecules biosynthesis and activation of antioxidant metabolism on callus cultures of .

摘要

背景

对外源氧化应激预处理茼蒿愈伤组织培养物可诱导过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、总酚、总黄酮、总蛋白以及选定的商业酚类化合物的产生和积累。

材料与方法

将从花梗外植体获得的愈伤组织培养物在含有10 mM H₂O₂或0 mM H₂O₂（对照组）的Murashige和Skoog培养基（MS）上培养10小时。通过LC-ESI-MS/MS分析在10 mM H₂O₂处理组和对照组的甲醇提取物中检测了20种酚类化合物（芹菜素、咖啡酸、对香豆酸、没食子酸、染料木黄酮、山奈酚、木犀草素、杨梅素、原花青素B1、槲皮素、芦丁水合物、香草酸、阿魏酸、水杨酸、芥子酸、绿原酸、橙皮苷、柚皮苷、迷迭香酸和异鼠李素）。

结果

主要的酚类化合物是绿原酸，其次是芦丁水合物、咖啡酸、木犀草素、异鼠李素、槲皮素、杨梅素、芹菜素、对香豆酸和山奈酚。在茼蒿的H₂O₂处理组和对照组中均未检测到没食子酸、染料木黄酮、原花青素B1、香草酸、芥子酸、橙皮苷和柚皮苷。估计的总酚含量顺序为H₂O₂处理组（285.36 μg/g干重）和对照组（220.79 μg/g干重）。仅在H₂O₂处理的愈伤组织材料中测定了山奈酚、杨梅素、槲皮素和异鼠李素的生物合成和积累。H₂O₂预处理明显显示出酶促和非酶促抗氧化活性的提高。最后，酚类积累与CAT、SOD、总酚、总黄酮和脯氨酸的综合活性之间存在显著正相关。