Maiborodin I V, Morozov V V, Anikeev A A, Figurenko N F, Maslov R V, Matveeva V A, Chastikin G A, Maiborodina V I
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
Bull Exp Biol Med. 2017 Aug;163(4):554-560. doi: 10.1007/s10517-017-3848-1. Epub 2017 Aug 29.
The peculiarities of tissue sclerosis after injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained with Vybrant CM-Dil cell membrane dye were studied by light microscopy with luminescence. The surgical intervention consisting in ligation of the great vein was followed by tissue sclerotic transformation caused by direct damage and chronic inflammation caused by the presence of slowly resorbed ligature. Injection of stromal cells after this intervention led to formation of more extensive scar. This can attest to the possibility of stromal cells differentiation into connective tissue cells, fibroblasts, and stimulation of proliferation and collagen synthesis by host fibroblasts. A decrease in the volume of dense fibrous connective tissue due to scar reorganization at latter terms cannot not excluded.
通过带发光的光学显微镜研究了注射用绿色荧光蛋白（GFP）基因转染并用Vybrant CM-Dil细胞膜染料染色的自体骨髓多能间充质基质细胞后组织硬化的特点。大静脉结扎的外科手术干预后，由于直接损伤以及缓慢吸收的结扎线存在导致的慢性炎症，引发了组织硬化转变。在此干预后注射基质细胞导致形成更广泛的瘢痕。这可以证明基质细胞分化为结缔组织细胞、成纤维细胞以及宿主成纤维细胞刺激增殖和胶原合成的可能性。后期由于瘢痕重组导致致密纤维结缔组织体积减少也不能排除。
