Maiborodin Igor, Lushnikova Elena, Klinnikova Marina, Klochkova Swetlana
Laboratory of Cell Biology and Cytology, Institute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution "Federal Research Center of Fundamental and Translational Medicine" of the Ministry of Science and Higher Education of the Russian Federation, Acad. Timakov st., 2, 630117 Novosibirsk, Russia.
Laboratory of Health Management Technologies, The Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch, Akademika Lavrenteva str. 8, 630090 Novosibirsk, Russia.
Life (Basel). 2021 Jan 18;11(1):66. doi: 10.3390/life11010066.
Changes in rat liver after resection and injection of autologous multipotent mesenchymal stromal cells of bone marrow origin (MSCs) transfected with the GFP gene and cell membranes stained with red-fluorescent lipophilic membrane dye were studied by light microscopy. It was found that after the introduction of MSCs into the damaged liver, their differentiation into any cells was not found. However, under the conditions of MSCs use, the number of neutrophils in the parenchyma normalizes earlier, and necrosis and hemorrhages disappear more quickly. It was concluded that the use of MSCs at liver resection for the rapid restoration of an organ is inappropriate, since the injected cells in vivo do not differentiate either into hepatocytes, into epithelial cells of bile capillaries, into endotheliocytes and pericytes of the vascular membranes, into fibroblasts of the scar or other connective tissue structures, or into any other cells present in the liver.
通过光学显微镜研究了大鼠肝脏在切除并注射经绿色荧光蛋白（GFP）基因转染的骨髓源性自体多能间充质基质细胞（MSCs）以及用红色荧光亲脂性膜染料染色的细胞膜后的变化。结果发现，将MSCs引入受损肝脏后，未发现它们分化为任何细胞。然而，在使用MSCs的条件下，实质内中性粒细胞的数量更早恢复正常，坏死和出血消失得更快。得出的结论是，在肝脏切除时使用MSCs来快速恢复器官是不合适的，因为体内注射的细胞既不会分化为肝细胞、胆小管上皮细胞、血管膜的内皮细胞和周细胞、瘢痕或其他结缔组织结构的成纤维细胞，也不会分化为肝脏中存在的任何其他细胞。
