Wang Long, Lin Qinlu, Yang Tao, Liang Ying, Nie Ying, Luo Yi, Shen Junjun, Fu Xiangjin, Tang Yiping, Luo Feijun
Laboratory of Molecular Nutrition, College of Food Science and Engineering, The Key Laboratory of Hunan Province for Special Medical Food, National Engineering Laboratory for Deep Processing of Rice and Byproducts, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
Department of Clinic Medicine, Xiangya School of Medicine, Central South University , Changsha, Hunan 410008, China.
J Agric Food Chem. 2017 Sep 27;65(38):8374-8385. doi: 10.1021/acs.jafc.7b03230. Epub 2017 Sep 19.
In Western countries and China, the dietary habit of high calories usually results in hyperlipidemia, which is closely associated with cardiovascular diseases. In the study, we investigated the antihyperlipidemic effect of oryzanol and its molecular mechanism in the high fat diet (HFD) mouse model. In total, 60 ICR mice were randomly divided into control group, HFD group, and HFD+Ory group. The mice from the HFD+Ory group were additionally fed with 100 mg/kg of oryzanol by intragastric administration. Our data indicated that oryzanol treatment for 10 weeks significantly reduced bodyweight, liver weight, and adipose tissues weight of the mice; lowered the contents of total cholesterol (TC), triglycerides (TG), and low density lipoprotein-cholesterol (LDL-C); and elevated high density lipoprotein-cholesterol (HDL-C) in the plasma of HFD mice. Compared with the HFD group, H&E staining showed that oryzanol treatment decreased the size of fat droplets of liver tissues and the size of adipocytes. Gene chip data found that oryzanol administration caused 32 genes to increase expressions while 60 genes had reduced expressions in the liver tissues of HFD mice. IPA software was used to analyze the protein interaction network and found that transcript factor NF-κB located in the central role of network, meaning NF-κB may have important function in the lipid-lowering effect of oryzanol. Western blotting and RT-qPCR confirmed that lipid metabolism-related gene expressions were obviously regulated by oryzanol administration. Oryzanol also inhibited expressions of inflammatory factor in the liver tissues of HDF mice. Taken together, our data indicate that oryzanol treatment can regulate lipid metabolism-related gene expressions and inhibit HDF-caused obesity in mice.
在西方国家和中国，高热量的饮食习惯通常会导致高脂血症，而高脂血症与心血管疾病密切相关。在本研究中，我们在高脂饮食（HFD）小鼠模型中研究了谷维素的降血脂作用及其分子机制。总共60只ICR小鼠被随机分为对照组、HFD组和HFD+谷维素组。HFD+谷维素组的小鼠通过灌胃额外给予100mg/kg的谷维素。我们的数据表明，谷维素治疗10周可显著降低小鼠的体重、肝脏重量和脂肪组织重量；降低高脂饮食小鼠血浆中总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）的含量；并升高高密度脂蛋白胆固醇（HDL-C）。与HFD组相比，苏木精-伊红（H&E）染色显示，谷维素治疗可减小肝脏组织中脂肪滴的大小和脂肪细胞的大小。基因芯片数据发现，给予谷维素导致高脂饮食小鼠肝脏组织中有32个基因表达增加，而60个基因表达减少。使用IPA软件分析蛋白质相互作用网络，发现转录因子NF-κB位于网络的中心位置，这意味着NF-κB可能在谷维素的降脂作用中具有重要功能。蛋白质免疫印迹法（Western blotting）和实时定量聚合酶链反应（RT-qPCR）证实，给予谷维素可明显调节脂质代谢相关基因的表达。谷维素还抑制了高脂饮食小鼠肝脏组织中炎症因子的表达。综上所述，我们的数据表明，谷维素治疗可调节脂质代谢相关基因的表达，并抑制高脂饮食引起的小鼠肥胖。
