Vandesmet Vivianne C S, Felipe Cícero F B, Kerntopf Marta R, Rolón Miriam, Vega Celeste, Coronel Cathia, Barbosa Andreza G R, Coutinho Henrique D M, Menezes Irwin R A
Faculdade Leão Sampaio, Juazeiro do Norte (CE), Brazil.
Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa (PB), Brazil.
Saudi J Biol Sci. 2017 Sep;24(6):1136-1141. doi: 10.1016/j.sjbs.2015.03.001. Epub 2015 Mar 7.
The evaluation of the leishmanicidal and trypanocidal activity of the hydroalcoholic extract of the bark of Mart. (EHCSR) was carried out to find an alternative treatment for parasitic diseases. EHCSR was prepared and used at four different concentrations (1000, 500, 250, 125 μg/mL) in assays for activity against Leishmania promastigotes using the species and and for trypanocidal activity using the epimastigotes of . We also tested EHCSR for cytotoxicity against adhered cultured Murine J774 fibroblasts. The tests were performed in triplicate, and the percent mortality of parasites, IC and percent toxicity were determined. With regard to anti-leishmania activity against , there was a mean mortality of 45% at all concentrations, and against , a substantial effect was seen at 1000 μg/mL with 56.38% mortality, where the IC values were 1338.76 and 987.35 μg/mL, respectively. Trypanocidal activity was notably high at 1000 μg/mL extract with 82.31% mortality of epimastigotes. Cytotoxicity at the highest extract concentrations of 500 and 1000 μg/mL was respectively 75.12% and 94.14%, with IC = 190.24 μg/mL. Despite that the extract has anti-parasitic activity, its substantial cytotoxicity against fibroblasts cells makes its systemic use nonviable as a therapeutic alternative.
对 Mart. 树皮的水醇提取物（EHCSR）的杀利什曼原虫和杀锥虫活性进行了评估，以寻找治疗寄生虫病的替代方法。制备了 EHCSR，并在针对利什曼原虫前鞭毛体的活性测定中使用四种不同浓度（1000、500、250、125 μg/mL），使用 和 物种，以及在针对 的上鞭毛体的杀锥虫活性测定中使用。我们还测试了 EHCSR 对粘附培养的小鼠 J774 成纤维细胞的细胞毒性。测试重复进行三次，并确定寄生虫的死亡率百分比、IC 和毒性百分比。关于对 的抗利什曼原虫活性，所有浓度下的平均死亡率为 45%，而对于 ，在 1000 μg/mL 时观察到显著效果，死亡率为 56.38%，其中 IC 值分别为 1338.76 和 987.35 μg/mL。杀锥虫活性在提取物浓度为 1000 μg/mL 时显著较高，上鞭毛体死亡率为 82.31%。提取物最高浓度 500 和 1000 μg/mL 时的细胞毒性分别为 75.12%和 94.14%，IC = 190.24 μg/mL。尽管该提取物具有抗寄生虫活性，但其对成纤维细胞的显著细胞毒性使其作为治疗替代品无法全身使用。
