Laboratory of Pharmacology and Molecular Chemistry, Regional University of Cariri, Crato, Brazil.
Pharm Biol. 2011 Dec;49(12):1265-70. doi: 10.3109/13880209.2011.589857. Epub 2011 Aug 16.
Stryphnodendron rotundifolium Mart. (Leguminosae), a tree in Northeast Brazil (Chapada do Araripe), is used in popular medicine to treat different processes such as inflammation and infectious diseases, mainly caused by bacterial pathogens.
This study determined the modulatory and antimicrobial activity of the hydroethanol extract of dried stem bark, the most used form of this natural product, as a remedy by the traditional communities, against standard and clinical isolates of Escherichia coli and Staphylococcus aureus.
The antibacterial and modulatory activities of the hydroalcoholic extract from the leaves were obtained by maceration/hydrodistillation method and assayed by microdilution.
In the microbiological assays, growth inhibition was demonstrated by this extract against the bacterial strains tested, with minimal inhibitory concentration (MIC) values of 512 μg/mL. However, when a subinhibitory concentration (MIC/8 = 64 μg/mL) was combined with conventional antimicrobial drugs (gentamicin, kanamycin, amikacin and neomycin), the extract showed a potentiating effect, reducing the MIC for all drugs assayed in a range between 312.5 and 2.4 μg/mL.
We indicate that the extract of S. rotundifolium showed potential synergistic antibiotic activity. With the results obtained, these extracts proved to be a promising source of antibacterial and modulatory agents.
Stryphnodendron rotundifolium Mart.（豆科），一种生长在巴西东北部（阿雷格里港高地）的乔木，被民间医学用于治疗多种疾病，如炎症和传染病，主要由细菌病原体引起。
本研究旨在测定干茎树皮水醇提取物的调节和抗菌活性，该提取物是该天然产物最常用的形式，作为传统社区的治疗方法，针对大肠杆菌和金黄色葡萄球菌的标准和临床分离株。
采用浸渍/水蒸馏法从叶中获得水醇提取物，并通过微量稀释法测定其抗菌和调节活性。
在微生物学检测中，该提取物对所测试的细菌菌株表现出抑制生长的作用，最小抑菌浓度（MIC）值为 512μg/mL。然而，当亚抑菌浓度（MIC/8=64μg/mL）与常规抗菌药物（庆大霉素、卡那霉素、阿米卡星和新霉素）联合使用时，提取物显示出增效作用，将所有检测药物的 MIC 值降低到 312.5 至 2.4μg/mL 之间。
我们表明，S. rotundifolium 的提取物表现出潜在的协同抗生素活性。基于这些结果，这些提取物被证明是具有前景的抗菌和调节药物来源。
