Department of Biological Chemistry, Laboratory of Natural Products, Program of Post-Graduation in Molecular Bioprospection, Regional University of Cariri, Street Cel. Antônio Luiz 1161, Pimenta, 63105-000 Crato-CE, Brazil.
Molecules. 2012 Jan 18;17(1):934-50. doi: 10.3390/molecules17010934.
Stryphnodendron rotundifolium is a phytotherapic used in the northeast of Brazil for the treatment of inflammatory processes which normally are associated with oxidative stress. Consequently, we have tested the antioxidant properties of hydroalcoholic (HAB) and aqueous extracts (AB) from the bark and aqueous extract (AL) from the leaves of Stryphnodendron rotundifolium to determine a possible association between antioxidant activity and the popular use of this plant. Free radical scavenger properties were assessed by the quenching of 1',1'-diphenil-2-picrylhydrazyl (DPPH) and the calculated IC(50) were: HAB = 5.4 ± 0.7, AB = 12.0 ± 2.6, and AL = 46.3 ± 12.3 µg/mL. Total phenolic contents were: HAB = 102.7 ± 2.8, AB = 114.4 ± 14.6, and AL = 93.8 ± 9.1 µg/mg plant). HPLC/DAD analyses indicated that gallic acid, catechin, rutin and caffeic acid were the major components of the crude extracts of S. rotundifolium. Plant extracts inhibited Fe(II)-induced lipid peroxidation in brain homogenates. Iron chelation was also investigated and only HBA exhibited a weak activity. Taken together, the results suggest that S. rotundifolium could be considered an effective agent in the prevention of diseases associated with oxidative stress.
轮环藤是一种植物药，在巴西东北部被用于治疗与氧化应激相关的炎症过程。因此，我们测试了轮环藤的树皮水醇提物（HAB）、水提物（AB）和叶水提物（AL）的抗氧化性能，以确定抗氧化活性与该植物的民间用途之间是否存在关联。通过淬灭 1',1'-二苯基-2-苦基肼基（DPPH）来评估自由基清除能力，并计算出的 IC（50）值分别为：HAB = 5.4 ± 0.7，AB = 12.0 ± 2.6，AL = 46.3 ± 12.3 µg/mL。总酚含量分别为：HAB = 102.7 ± 2.8，AB = 114.4 ± 14.6，AL = 93.8 ± 9.1 µg/mg 植物）。HPLC/DAD 分析表明，没食子酸、儿茶素、芦丁和咖啡酸是轮环藤粗提取物的主要成分。植物提取物抑制了脑匀浆中 Fe（II）诱导的脂质过氧化。还研究了铁螯合作用，只有 HBA 表现出较弱的活性。综上所述，这些结果表明轮环藤可能被认为是预防与氧化应激相关疾病的有效药物。
