El-Sherbini El-Said, El-Sayed Gehad, El Shotory Rehab, Gheith Nervana, Abou-Alsoud Mohamed, Harakeh Steve Mustapha, Karrouf Gamal I
Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Egypt.
Public Administration Departments, Faculty of Economic and Administration, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Saudi J Biol Sci. 2017 Sep;24(6):1410-1417. doi: 10.1016/j.sjbs.2016.08.010. Epub 2016 Sep 1.
Lead intoxication has been a major health hazard in humans. It affects people at all ages. Its toxicity is associated with various organs of the body and affects different metabolic pathways. Based on histological data, l-carnitine reduced the severity of tissue damage produced as a result of exposure of rats to lead acetate. The main objective of this study was to evaluate the underlying mechanism of protection offered by l-carnitine against lead acetate intoxication using male Sprague-Dawley rats. Forty male Sprague-Dawley rats were randomly divided into four groups with ten rats in each. The first group (G1) served as the control group and animals received standard diet only. The second group (G2) received lead acetate in their diet. The third group (G3) was the l-carnitine treated group and received the normal standard diet supplemented with l-carnitine. While the fourth group (G4) had a diet supplemented with both lead acetate and l-carnitine. At the end of each experiment, blood (serum and whole blood) were collected from each animal and analyzed for the following parameters: serum testosterone levels, serum nitric oxide and serum malondialdehyde. This is in addition to looking at the enzymatic activities of two important enzymes (superoxide dismutase and catalase) and on (glutathione reductase) which are indicative of the antioxidant activities in the whole blood. The results indicated that l-carnitine will counteract the undesirable effects of lead intoxication. It exerted its antioxidant potential by reducing the production of ROS and scavenging free radicals by maintaining and protecting the level of the of antioxidant enzymes SOD, CAT and glutathione peroxidase. l-Carnitine may play an important role in reversing the undesirable effects of lead intoxication. Future studies should be conducted to see whether such an effect is applicable in humans exposed to lead poising.
铅中毒一直是人类面临的主要健康危害。它影响各个年龄段的人群。其毒性与人体的各个器官相关，并影响不同的代谢途径。基于组织学数据，左旋肉碱降低了大鼠接触醋酸铅后产生的组织损伤严重程度。本研究的主要目的是使用雄性Sprague-Dawley大鼠评估左旋肉碱对醋酸铅中毒提供保护作用的潜在机制。40只雄性Sprague-Dawley大鼠被随机分为四组，每组10只。第一组（G1）作为对照组，动物仅接受标准饮食。第二组（G2）在饮食中摄入醋酸铅。第三组（G3）是左旋肉碱处理组，接受补充了左旋肉碱的正常标准饮食。而第四组（G4）的饮食中同时补充了醋酸铅和左旋肉碱。在每个实验结束时，从每只动物采集血液（血清和全血），并分析以下参数：血清睾酮水平、血清一氧化氮和血清丙二醛。此外，还要观察两种重要酶（超氧化物歧化酶和过氧化氢酶）以及（谷胱甘肽还原酶）的酶活性，这些酶活性可指示全血中的抗氧化活性。结果表明，左旋肉碱将抵消铅中毒的不良影响。它通过减少活性氧的产生并通过维持和保护抗氧化酶超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平来清除自由基，从而发挥其抗氧化潜力。左旋肉碱可能在逆转铅中毒的不良影响方面发挥重要作用。未来应进行研究，以确定这种效果是否适用于铅中毒的人类。
