Wang Yihan, He Jie, Li Jiao, Lu Tong, Li Yong, Ma Wenjuan, Zhang Limin, Zhou Zhongxing, Zhao Huijuan, Gao Feng
College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China.
Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin 300072, China.
Biomed Opt Express. 2017 Jul 24;8(8):3778-3795. doi: 10.1364/BOE.8.003778. eCollection 2017 Aug 1.
Several attempts to achieve the quantitative photoacoustic tomography (q-PAT) have been investigated using point sources or a single-angle wide-field illumination. However, these schemes normally suffer from low signal-to-noise ratio (SNR) or poor quantification in imaging applications on large-size domains, due to the limitation of ANSI-safety incidence and incompleteness in the data acquisition. We herein present a q-PAT implementation that uses multi-angle light-sheet illuminations and calibrated recovering-and-averaging iterations. The scheme can obtain more complete information on the intrinsic absorption from the multi-angle illumination mode, and collect SNR-boosted photoacoustic signals in the selected planes from the wide-field light-sheet excitation. Therefore, the sliced absorption maps over whole body of small-animals can be recovered in a measurement-flexible, noise-robust and computation-economic way. The proposed approach is validated by phantom, and experiments, exhibiting promising performances in image fidelity and quantitative accuracy for practical applications.
已经使用点光源或单角度宽场照明对实现定量光声断层扫描（q-PAT）进行了多次尝试。然而，由于ANSI安全入射的限制和数据采集的不完整性，这些方案在大尺寸区域的成像应用中通常存在信噪比（SNR）低或量化效果差的问题。我们在此提出一种q-PAT实现方法，该方法使用多角度光片照明以及校准的恢复和平均迭代。该方案可以从多角度照明模式获得关于固有吸收的更完整信息，并从宽场光片激发中在选定平面收集信噪比增强的光声信号。因此，可以以测量灵活、抗噪声且计算经济的方式恢复小动物全身的切片吸收图。所提出的方法通过模型和实验得到验证，在实际应用的图像保真度和定量准确性方面表现出有前景的性能。
