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圣哈辛托河垃圾坑下游受二噁英污染沉积物中DF05的基因组揭示了大量芳香族降解基因决定因素。

Genome of DF05 from dioxin contaminated sediment downstream of the San Jacinto River waste pits reveals a broad array of aromatic degradation gene determinants.

作者信息

Iyer Rupa, Iken Brian, Damania Ashish

机构信息

Center for Life Sciences Technology, Engineering Technology, University of Houston, 300 Technology Building, Houston, TX 77204, USA.

Department of Pediatrics-Tropical Medicine, Baylor College of Medicine, Houston, TX, USA.

出版信息

Genom Data. 2017 Aug 17;14:40-43. doi: 10.1016/j.gdata.2017.07.011. eCollection 2017 Dec.

Abstract

. DF05 is a Gram negative, motile, aerobic, rod-shaped and psychrotrophic bacterium that was isolated from contaminated San Jacinto River sediment near River Terrace Park in Channelview, Texas. The draft genome of strain DF05 consists of a total of 192 contigs assembled at the scaffold level totaling 6,487,527 bp and encoding for 5862 functional proteins, 1116 of which are annotated as hypothetical proteins. The bacterial chromosome has a GC content of 65.15% and contains 22 rRNA and 70 tRNA loci. In addition, approximately 142 proteins localized on the bacterial chromosome are associated with metabolism of aromatic compounds. A single plasmid approximately 95 kb in size was also detected carrying copies of RNA genes and multiple phage assembly proteins.

摘要

DF05是一种革兰氏阴性、具运动性、需氧、杆状且嗜冷的细菌,它是从得克萨斯州钱尼尔维尤市河滨台地公园附近受污染的圣哈辛托河沉积物中分离出来的。菌株DF05的基因组草图由总共192个重叠群组成,这些重叠群在支架水平上组装,总计6,487,527 bp,编码5862个功能蛋白,其中1116个被注释为假设蛋白。细菌染色体的GC含量为65.15%,包含22个rRNA和70个tRNA基因座。此外,大约142个位于细菌染色体上的蛋白质与芳香族化合物的代谢有关。还检测到一个大小约为95 kb的单一质粒,它携带RNA基因拷贝和多种噬菌体组装蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5147/5565767/cb49ec0e3485/gr1.jpg

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