School of Information Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang, People's Republic of China.
School of Pharmacy, Shihezi University, Shihezi, 832000, Xinjiang, People's Republic of China.
Appl Biochem Biotechnol. 2018 Mar;184(3):763-776. doi: 10.1007/s12010-017-2574-5. Epub 2017 Aug 30.
Coreopsis tinctoria flowering tops (CTFs) is a popular health product as herbal tea or as a traditional medicinal herb that is rich in saponins and exerts substantial biological activity. In this study, an ultrasound-assisted aqueous two-phase system (ATPS) was utilized to extract total saponins from CTFs and optimize the extraction process by response surface methodology. Moreover, the nitric oxide and nitrite scavenging capability, and N-nitrosamine formation inhibitory activity of total saponins were evaluated. Results showed that the optimal conditions for total saponins were 37.76% (w/w) ammonium sulfate and 35.62% (w/w) ethanol in ATPS coupled with ultrasonic-assisted extraction. Under the optimal conditions, the maximum yield of total saponins of 33.4 g/kg can be obtained from the CTFs raw material. The nitric oxide radical scavenging, nitrite scavenging, and N-nitrosamine inhibitory activities (SC) were 287.92 ± 7.42, 191.63 ± 7.69, and 1787.4 ± 51.26 μg/mL, respectively. The total saponins has a certain nitric oxide and nitrite scavenging capability, and N-nitrosamine formation inhibitory activity in vitro. Given these activities, research on saponins from CTFs provides profound and lasting implications for the novel applications of C. tinctoria.
金鸡菊开花顶部（CTFs）是一种流行的健康产品，作为草药茶或作为传统草药，富含皂甙，并发挥大量的生物活性。在这项研究中，利用超声辅助双水相体系（ATPS）从 CTFs 中提取总皂甙，并通过响应面法优化提取工艺。此外，还评价了总皂甙的一氧化氮和亚硝酸盐清除能力以及亚硝胺形成抑制活性。结果表明，ATPS 与超声辅助提取相结合时，总皂甙的最佳条件为 37.76%（w/w）硫酸铵和 35.62%（w/w）乙醇。在最佳条件下，从 CTFs 原料中可获得 33.4 g/kg 的总皂甙最大产量。一氧化氮自由基清除、亚硝酸盐清除和亚硝胺抑制活性（SC）分别为 287.92±7.42μg/ml、191.63±7.69μg/ml和 1787.4±51.26μg/ml。总皂甙在体外具有一定的一氧化氮和亚硝酸盐清除能力以及亚硝胺形成抑制活性。鉴于这些活性，对 CTFs 皂甙的研究为 C. tinctoria 的新应用提供了深刻而持久的意义。
