Rigolin Maria Sílvia M, de Avila Erica Dorigatti, Basso Fernanda G, Hebling Josimeri, de S Costa Carlos Alberto, Mollo Junior Francisco de Assis
Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, Univ Estadual Paulista - UNESP. Araraquara, São Paulo, Brazil.
Department of Physiology and Pathology, School of Dentistry at Araraquara, Univ Estadual Paulista - UNESP. Araraquara, São Paulo, Brazil.
Microsc Res Tech. 2017 Dec;80(12):1304-1309. doi: 10.1002/jemt.22941. Epub 2017 Aug 30.
For the long-term success of implants, it is necessary to achieve a direct contact between the implant and the subjacent bone. To avoid bacterial penetration that could adversely affect the initial wound healing as well as the long-term behavior of the implants, an early tissue barrier must form that is able to protect the biological peri-implant structures. Given the need of an effective tissue early barrier around dental implants, the present study evaluated, in vitro, the influence of physical and chemical characteristics of two implant abutment surfaces on gingival epithelial cells (OBA-9) adhesion. To this end, titanium (Ti) and zirconia (ZrO ) disk-shaped specimens were used mimicking the abutment components surfaces, while bovine enamel (BE) and glass cover slips (GCS) disks served as positive and negative controls, respectively. Roughness and surface free energy (SFE) of all materials were evaluated previously to cellular adhesion step. In sequence, the effect of each material on cells morphology and viability was analyzed after 1 and 24 hr. The results showed that roughness and SFE had no effect on the cell viability data or on their interaction (p = .559), independent of a post-contact analysis of 1 or 24 hr. However, cells attachment and spreading increased after 24 hr on Ti and ZrO than BE, corresponding to the highest SFE values. SFE appears to be an important property interfering on the quality of the soft tissue surrounding dental implants. These data can be considered a trigger point for developing new material surfaces.
为了使种植体长期成功，种植体与下方骨组织实现直接接触是必要的。为避免细菌侵入对初始伤口愈合以及种植体的长期性能产生不利影响，必须形成早期组织屏障以保护种植体周围的生物结构。鉴于牙种植体周围需要有效的早期组织屏障，本研究在体外评估了两种种植体基台表面的物理和化学特性对牙龈上皮细胞（OBA - 9）黏附的影响。为此，使用钛（Ti）和氧化锆（ZrO）圆盘形标本模拟基台部件表面，而牛牙釉质（BE）和玻璃盖玻片（GCS）圆盘分别作为阳性和阴性对照。在细胞黏附步骤之前，先评估了所有材料的粗糙度和表面自由能（SFE）。接着，在1小时和24小时后分析了每种材料对细胞形态和活力的影响。结果表明，粗糙度和SFE对细胞活力数据或它们之间的相互作用没有影响（p = 0.559），与1小时或24小时的接触后分析无关。然而，在Ti和ZrO上培养24小时后，细胞的附着和铺展比在BE上增加，这与最高的SFE值相对应。SFE似乎是影响牙种植体周围软组织质量的一个重要特性。这些数据可被视为开发新材料表面的触发点。
