[不同等离子体处理的氧化锆对人牙龈成纤维细胞黏附行为的影响]
[Effect of different plasma treated zirconia on the adhensive behaviour of human gingival fibroblasts].
作者信息
Zheng M, Zhan L L, Liu Z Q, Li H P, Tan J G
机构信息
Department of Stomatology, Peking University Third Hospital, Beijing 100191, China.
Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2019 Apr 18;51(2):315-320. doi: 10.19723/j.issn.1671-167X.2019.02.022.
OBJECTIVE
To evaluate the effect of different cold atmospheric plasma (CAP) treatment on the surface chemical and physical properties of zirconia and adhensive behaviour of human gingival fibroblasts (HGFs) cultured on zirconia disks.
METHODS
The zirconia disks were divided into four groups and treated using helium, argon and mixture of argon and oxygen cold atmospheric plasma for 90 s or left untreated. The surface morphology, wettability and chemical elements were analyzed right after treatment. Human gingival fibroblasts were grown from biopsies obtained from a periodontally healthy human subject during periodontal surgery. HGFs were seeded on zirconia disk, and cells density was measured at the time point of 3 hours. Indirect immunofluorescence (IIF) was performed for morphometric examination at the time point of 3 hours.
RESULTS
The crystallographic structure of zirconia was analyzed previously and the results suggested that it fitted the properties of zirconium yttrium oxide. After helium, argon and mixture of argon and oxygen cold atmospheric plasma treatment, the surface morphology and roughness of zirconia disks remained the same. The contact angle of zirconia decreased significantly(P<0.05)after CAP treatment: from 68.38° to 17.90°. After different CAP plasmas treatment, the atomic percentage of carbon on the outermost surface of the three groups decreased, as did the surface C/O ratio. The surface C/O ratio of zirconia decreased from 1.07 to 0.33. Fibroblasts density increased on CAP treated disks, especially the ones treated by mixture of argon and oxygen CAP(P<0.05). Cells of the three CAP plasma treatment groups spread better and had more protrusions, as well as larger surficial areas.
CONCLUSION
Based on the results of this study after being treated by different kinds of CAP plasmas for 90 s, the surface wettability increased and the elements changed significantly with no changes in the tomography and roughness of the materials. The CAP treatment enhances the adhensive behavior of fibroblasts on zirconia by increasing the oxygen functional groups and promoting the cell density. Wettability of zirconia, an important physicochemical property, has a vital influence on the cell behaviors.
目的
评估不同冷大气等离子体（CAP）处理对氧化锆表面化学和物理性质以及在氧化锆盘上培养的人牙龈成纤维细胞（HGFs）黏附行为的影响。
方法
将氧化锆盘分为四组，分别用氦气、氩气以及氩气和氧气的混合气体进行冷大气等离子体处理90秒，或不进行处理。处理后立即分析其表面形态、润湿性和化学元素。从一名牙周健康的人类受试者在牙周手术期间获取的活检组织中培养人牙龈成纤维细胞。将HGFs接种在氧化锆盘上，并在3小时时间点测量细胞密度。在3小时时间点进行间接免疫荧光（IIF）检测以进行形态学检查。
结果
先前已分析氧化锆的晶体结构，结果表明其符合钇稳定氧化锆的特性。经过氦气、氩气以及氩气和氧气的混合气体冷大气等离子体处理后，氧化锆盘的表面形态和粗糙度保持不变。CAP处理后氧化锆的接触角显著降低（P<0.05）：从68.38°降至17.90°。经过不同的CAP等离子体处理后，三组最外层表面的碳原子百分比降低，表面C/O比也降低。氧化锆的表面C/O比从1.07降至0.33。CAP处理的盘上成纤维细胞密度增加，尤其是经氩气和氧气混合CAP处理的盘（P<0.05）。三个CAP等离子体处理组的细胞铺展更好，有更多突起，且表面积更大。
结论
基于本研究结果，在经过不同种类的CAP等离子体处理90秒后，表面润湿性增加，元素发生显著变化，而材料的断层扫描和粗糙度无变化。CAP处理通过增加氧官能团和促进细胞密度来增强成纤维细胞在氧化锆上的黏附行为。氧化锆的润湿性作为一种重要的物理化学性质，对细胞行为有至关重要的影响。
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