Alonso María Rosario, Peralta Ignacio, Monti Demian, Martino Renzo, Anesini Claudia
Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de la Química y Metabolismo del Fármaco (IQUIMEFA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Phytother Res. 2017 Nov;31(11):1708-1714. doi: 10.1002/ptr.5897. Epub 2017 Aug 31.
Larrea divaricata Cav. (Zygophyllaceae) is a South American plant widely distributed in Argentina that is used in folk medicine to treat inflammatory diseases. The aqueous extract is known to have well-documented biological activities such as antitumour, immunomodulatory, antimicrobial, antiinflammatory and antioxidant. However, its stability in gastrointestinal fluids is unknown. The latter is an important factor to assure the bioavailability of plant extracts intended to be administered via the oral route. The aim of this work was to study the stability of a lyophilized aqueous extract of L. divaricata compressed as a pill. To this end, the main polyphenol compound found in the extract, that is, the nordihydroguaiaretic acid, the total polyphenols and flavonoids content and the antioxidant activity such as diphenylpicrylhydrazyl scavenger activity and reducing power were assayed after subjecting the extract to different incubation times in simulated digestive fluids. The HPLC and spectroscopic methods were employed. Although the levels of polyphenols and flavonoids decreased upon incubation in gastric and intestinal fluids, the extract maintained its antioxidant activity related to the presence of nordihydroguaiaretic acid. These results are promising and encourage the potential use of the extract by the oral route as a supplement or phytomedicine with antioxidant activity. Copyright © 2017 John Wiley & Sons, Ltd.
拉雷亚二歧银胶菊（Larrea divaricata Cav.，蒺藜科）是一种广泛分布于阿根廷的南美植物，在民间医学中用于治疗炎症性疾病。已知其水提取物具有抗肿瘤、免疫调节、抗菌、抗炎和抗氧化等充分记录的生物活性。然而，其在胃肠液中的稳定性尚不清楚。后者是确保旨在通过口服途径给药的植物提取物生物利用度的一个重要因素。这项工作的目的是研究制成药丸的拉雷亚二歧银胶水冻干提取物的稳定性。为此，在将提取物置于模拟消化液中不同孵育时间后，测定提取物中发现的主要多酚化合物即去甲二氢愈创木酸、总多酚和黄酮类化合物含量以及抗氧化活性，如二苯基苦味酰基自由基清除活性和还原能力。采用了高效液相色谱法和光谱法。尽管在胃液和肠液中孵育后多酚和黄酮类化合物的水平有所下降，但提取物因其去甲二氢愈创木酸的存在而保持了其抗氧化活性。这些结果很有前景，并鼓励通过口服途径将该提取物作为具有抗氧化活性的补充剂或植物药进行潜在应用。版权所有© 2017约翰·威利父子有限公司。
