Luo Mu-Tan, Huang Chao, Chen Xue-Fang, Huang Qian-Lin, Qi Gao-Xiang, Tian Lan-Lan, Xiong Lian, Li Hai-Long, Chen Xin-De
a Key Laboratory of Renewable Energy , Chinese Academy of Sciences , Guangzhou , P. R. China.
b Guangzhou Institute of Energy Conversion , Chinese Academy of Sciences , Guangzhou , P. R. China.
Prep Biochem Biotechnol. 2017 Nov 26;47(10):1025-1031. doi: 10.1080/10826068.2017.1373290. Epub 2017 Aug 31.
Biomass acid hydrolysate of oleaginous yeast Trichosporon cutaneum after microbial oil extraction was applied as substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus (also named as Gluconacetobacter xylinus previously) for the first time. BC was synthesized in static culture for 10 days, and the maximum BC yield (2.9 g/L) was got at the 4th day of fermentation. Most carbon sources in the substrate (glucose, mannose, formic acid, acetic acid) can be utilized by K. xylinus. The highest chemical oxygen demand (COD) removal (40.7 ± 3.0%) was obtained at the 6th day of fermentation, and then the COD increased possibly due to the degradation of BC. The highest BC yield on COD consumption was 38.7 ± 4.0% (w/w), suggesting that this is one efficient bioconversion for BC production. The BC structure was affected little by the substrate by comparison with that generated in classical HS medium using field-emission scanning electron microscope (FE-SEM), Fourier transform infrared, and X-ray diffraction. Overall, this technology can both solve the issue of waste oleaginous yeast biomass and produce valuable biopolymer (BC).
微生物油脂提取后，产油酵母皮状丝孢酵母的生物质酸水解产物首次被用作木醋杆菌（以前也称为木糖醋酸杆菌）生产细菌纤维素（BC）的底物。在静态培养中合成BC持续10天，在发酵第4天获得了最高BC产量（2.9 g/L）。底物中的大多数碳源（葡萄糖、甘露糖、甲酸、乙酸）都能被木醋杆菌利用。在发酵第6天获得了最高的化学需氧量（COD）去除率（40.7±3.0%），然后COD可能由于BC的降解而增加。基于COD消耗的最高BC产量为38.7±4.0%（w/w），表明这是一种高效的BC生产生物转化方法。通过场发射扫描电子显微镜（FE-SEM）、傅里叶变换红外光谱和X射线衍射比较，与在经典HS培养基中产生的BC结构相比，底物对BC结构影响较小。总体而言，该技术既能解决废弃产油酵母生物质的问题，又能生产有价值的生物聚合物（BC）。
