Dhotre Dhiraj Prakash, Rajabal Vaheesan, Sharma Avinash, Kulkarni Girish J, Prakash Om, Vemuluri Venkata Ramana, Joseph Neetha, Rahi Praveen, Shouche Yogesh S
Microbial Culture Collection, National Centre for Cell Science, Pune, Maharashtra 411007, India.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2766-2772. doi: 10.1099/ijsem.0.002019.
The taxonomic positions of Microterricola viridarii JCM 15926T, Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high sequence similarity (99.2 -99.9 %). A dendrogram constructed based on the protein spectra generated by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy also displayed close clustering of these three strains. The fatty acid profiles of three strains were very similar to each other and contained branched fatty acids (anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0) as the predominant cellular fatty acids. The polar lipid profiles of the three stains were similar and consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as major polar lipids and an unknown lipid. Comparisons of morphological, chemotaxonomic and physiological data of Microterricola viridarii JCM 15926T, Leifsonia pindariensis JCM 15132T and Phycicola gilvus DSM 18319T are in agreement with the features of a common genus. DNA-DNA hybridization data generated during this study showed less than 70 % reassociation value with each other indicating that they are different at species level. Based on the present study, we conclude that Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T should be reclassified under the genus Microterricola, since this genus has the nomenclatural priority, and reclassified as Microterricolagilva comb. nov. (type strain SSWW-21T=DSM 18319T=KCTC 19185T=JCM 30550T) and Microterricolapindariensis comb. nov. (type strain PON10T=LMG 24222T=JCM 15132T=MTCC9128T). An emended description of the genus Microterricola is also presented.
对绿色微土杆菌JCM 15926T、吉尔维藻杆菌DSM 18319T和平达里利夫松氏菌JCM 15132T的分类地位进行了重新审视。系统发育分析和16S rRNA基因序列相似性表明，这三株菌彼此密切相关，形成了一个具有高序列相似性（99.2 - 99.9%）的单系聚类。基于基质辅助激光解吸/电离飞行时间质谱产生的蛋白质谱构建的树状图也显示这三株菌紧密聚类。这三株菌的脂肪酸谱彼此非常相似，并且含有支链脂肪酸（anteiso-C15 : 0、anteiso-C17 : 0和iso-C16 : 0）作为主要的细胞脂肪酸。这三株菌的极性脂质谱相似，由双磷脂酰甘油、磷脂酰甘油和磷脂酰胆碱作为主要极性脂质以及一种未知脂质组成。对绿色微土杆菌JCM 15926T、平达里利夫松氏菌JCM 15132T和吉尔维藻杆菌DSM 18319T的形态、化学分类和生理数据的比较与一个共同属的特征相符。本研究期间产生的DNA-DNA杂交数据显示它们彼此之间的重结合值低于70%，表明它们在种水平上不同。基于本研究，我们得出结论，吉尔维藻杆菌DSM 18319T和平达里利夫松氏菌JCM 15132T应重新分类到微土杆菌属，因为该属具有命名优先权，并重新分类为吉尔维微土杆菌（Microterricola gilva）新组合（模式菌株SSWW-21T = DSM 18319T = KCTC 19185T = JCM 30550T）和平达里微土杆菌（Microterricola pindariensis）新组合（模式菌株PON10T = LMG 24222T = JCM 15132T = MTCC9128T）。还给出了微土杆菌属的修订描述。
