Wu Hao, Liu Bin, Shao Yuanyuan, Ou Xiaoyun, Huang Fuchang
Institute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning, PR China.
Int J Syst Evol Microbiol. 2018 Feb;68(2):602-608. doi: 10.1099/ijsem.0.002551.
A novel thermophilic actinomycete, designated strain 3-12X, was isolated from mushroom compost in Guangxi University, Nanning, China. The novel isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were glucose and ribose. The predominant menaquinones were MK-9(H4) and MK-9(H6). The polar phospholipids were diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, ninhydrin-positive phosphoglycolipids and glycolipids. Major fatty acids were so-C16 : 0 and C17 : 0. The G+C content of the genomic DNA was 74.6 %. The 16S rRNA gene sequence analysis showed that the closest phylogenetic neighbour of strain 3-12X was Thermomonospora chromogena ATCC 43196 (97.0 %), other closely related strains all belonged to the family Streptosporangiaceae and showed more than 6 % divergence. The chemotaxonomic characteristics of strain 3-12X were significantly different from Thermomonospora chromogena ATCC 43196 and DNA-DNA hybridization showed low relatedness (48.6-55.6 %) between them, so they should be different species. Thermomonospora chromogena was removed from the genus Thermomonospora by Zhang et al. 1998 on the basis of phylogenetic, chemotaxonomic and phenotypic evidence, but its taxonomic position remains uncertain. Based on the phenotypic and phylogenetic data, strain 3-12X represents a novel species in a new genus in the family Streptosporangiaceae. The name Thermostaphylospora griseoalba gen. nov., sp. nov. is proposed. The type strain of Thermostaphylospora grisealba is 3-12X (=DSM 46781=CGMCC 4.7160). We also propose transferring Thermomonospora chromogenaZhang et al. 1998 to Thermostaphylospora chromogena comb. nov. (type strain ATCC 43196=JCM 6244).
从中国南宁广西大学的蘑菇堆肥中分离出一株新型嗜热放线菌，命名为3-12X菌株。该新分离菌株含有内消旋二氨基庚二酸作为诊断性二氨基酸，全细胞糖为葡萄糖和核糖。主要的甲基萘醌为MK-9(H4)和MK-9(H6)。极性磷脂为双磷脂酰甘油、羟基磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、茚三酮阳性磷酸糖脂和糖脂。主要脂肪酸为so-C16 : 0和C17 : 0。基因组DNA的G+C含量为74.6 %。16S rRNA基因序列分析表明，3-12X菌株最接近的系统发育邻居是嗜色栖热单孢菌ATCC 43196（相似度为97.0 %），其他密切相关菌株均属于链孢囊菌科，且差异超过6 %。3-12X菌株的化学分类特征与嗜色栖热单孢菌ATCC 43196显著不同，DNA-DNA杂交显示它们之间的相关性较低（48.6-55.6 %），因此它们应为不同的物种。1998年，张等人基于系统发育、化学分类和表型证据将嗜色栖热单孢菌从栖热单孢菌属中移除，但其分类地位仍不确定。基于表型和系统发育数据，3-12X菌株代表链孢囊菌科一个新属中的一个新物种。提出新属名嗜热葡萄孢菌属（Thermostaphylospora），新种名灰白嗜热葡萄孢菌（Thermostaphylospora griseoalba）。灰白嗜热葡萄孢菌的模式菌株为3-12X（=DSM 46781=CGMCC 4.7160）。我们还建议将张等人1998年命名的嗜色栖热单孢菌转移至嗜热葡萄孢菌属，新组合名为嗜色嗜热葡萄孢菌（Thermostaphylospora chromogena）（模式菌株ATCC 43196=JCM 6244）。
