Kowall N W, Ferrante R J, Beal M F, Richardson E P, Sofroniew M V, Cuello A C, Martin J B
Neuroscience. 1987 Mar;20(3):817-28. doi: 10.1016/0306-4522(87)90242-9.
Neuropeptide Y and somatostatin immunoreactive neurons and processes were examined in human striatum using both immunofluorescence and avidin biotin immunoperoxidase methods. Reduced nicotinamide adenine dinucleotide phosphate diaphorase activity was histochemically determined by the reduction of nitro blue tetrazolium. Immunofluorescence using a monoclonal anti-somatostatin antibody and a polyclonal anti-neuropeptide Y antibody, followed by diaphorase histochemistry, showed that these three neurochemical markers are co-localized in a single population of medium-sized aspiny intrinsic neurons. Cells were evenly distributed in clusters throughout the striatum, but fiber density was higher in the nucleus accumbens and ventromedial regions of the caudate and putamen. Double-stained reduced nicotinamide adenine dinucleotide phosphate diaphorase-acetylcholinesterase sections demonstrated that these neurons are located in zones of high acetylcholinesterase activity, often at the interface of these zones with regions of low enzyme activity. These biochemically distinctive neurons are uniquely situated to modulate activity between striatal compartments. Our findings provide new information about the modular organization of the striatum and extend these observations in human brain.
利用免疫荧光和抗生物素蛋白生物素免疫过氧化物酶方法，对人类纹状体中的神经肽Y和生长抑素免疫反应性神经元及突起进行了检测。通过硝基蓝四氮唑的还原，组织化学测定了还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶活性。使用单克隆抗生长抑素抗体和多克隆抗神经肽Y抗体进行免疫荧光，随后进行黄递酶组织化学分析，结果表明这三种神经化学标志物共定位于单个中等大小的无棘固有神经元群体中。细胞在整个纹状体中呈簇状均匀分布，但伏隔核以及尾状核和壳核的腹内侧区域的纤维密度较高。双重染色的还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶 - 乙酰胆碱酯酶切片显示，这些神经元位于高乙酰胆碱酯酶活性区域，通常位于这些区域与低酶活性区域的交界处。这些具有独特生化特性的神经元处于独特位置，可调节纹状体各部分之间的活动。我们的研究结果提供了有关纹状体模块化组织的新信息，并扩展了在人类大脑中的这些观察结果。
