Hara M, Akaike A, Sasa M, Takaori S
Neurosci Res. 1987 Apr;4(4):279-90. doi: 10.1016/0168-0102(87)90044-7.
Microiontophoretic studies were performed to elucidate the acute effects of methamphetamine on the nucleus accumbens (Acc) neurons receiving input from the parafascicular nucleus (Pf) of the thalamus using rats anesthetized with chloral hydrate. Spike generation upon Pf stimulation was inhibited by conditioning stimuli applied to the ventral tegmental area (VTA), which is rich in dopamine-containing neurons, and by iontophoretic application of methamphetamine as well as dopamine. The VTA-, methamphetamine- and dopamine-induced inhibition of the spikes elicited by Pf stimulation was antagonized during simultaneous application of haloperidol. Glutamate-induced firing was also inhibited during iontophoretic application of methamphetamine and dopamine in neurons receiving input from the Pf, and the inhibition was blocked by simultaneously applied haloperidol. In the reserpine-treated animals, however, the Pf-induced spikes were not affected by methamphetamine, but inhibited by dopamine. These results indicate that methamphetamine inhibits the Acc neurons receiving input from the Pf, probably by releasing dopamine from dopaminergic nerve terminals from the VTA.
采用水合氯醛麻醉的大鼠，进行微离子透入研究以阐明甲基苯丙胺对伏隔核（Acc）中接受来自丘脑束旁核（Pf）输入的神经元的急性作用。向富含多巴胺能神经元的腹侧被盖区（VTA）施加条件刺激，以及通过离子透入法施加甲基苯丙胺和多巴胺，均可抑制Pf刺激时的峰电位产生。在同时应用氟哌啶醇时，VTA、甲基苯丙胺和多巴胺诱导的对Pf刺激引发的峰电位的抑制作用被拮抗。在接受Pf输入的神经元中，离子透入应用甲基苯丙胺和多巴胺时，谷氨酸诱导的放电也受到抑制，且该抑制作用被同时应用的氟哌啶醇阻断。然而，在利血平处理的动物中，Pf诱导的峰电位不受甲基苯丙胺影响，但受多巴胺抑制。这些结果表明，甲基苯丙胺可能通过从VTA的多巴胺能神经末梢释放多巴胺来抑制接受Pf输入的Acc神经元。
