Kent R B, Fallows D A, Geissler E, Glaser T, Emanuel J R, Lalley P A, Levenson R, Housman D E
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5369-73. doi: 10.1073/pnas.84.15.5369.
We have made use of a panel of mouse-hamster somatic cell hybrids and restriction fragment length polymorphisms between two mouse species (Mus musculus and Mus spretus) to determine the chromosomal localization of genes encoding the alpha and beta subunits of the Na,K-ATPase (Na+,K+-activated ATP phosphohydrolase, EC 3.6.1.3). DNA probes for three distinct isoforms of the Na,K-ATPase alpha subunit mapped to three different mouse chromosomes: the alpha 1 gene (Atpa-1) cosegregated with the Egf gene on chromosome 3; alpha 2 (Atpa-2) with the cytochrome P-450PB gene family/coumarin hydroxylase locus on chromosome 7; alpha 3 (Atpa-3) with the alpha-spectrin gene on chromosome 1. The Na,K-ATPase beta-subunit gene (Atpb) mapped to the same region of chromosome 1, but it was not tightly linked to the Atpa-3 gene. These results indicate that three isoforms of the Na,K-ATPase alpha subunit are encoded by three distinct genes. The dispersion of Na,K-ATPase genes suggests that their expression is not likely to be controlled by a common cis-acting regulatory element.
我们利用一组小鼠 - 仓鼠体细胞杂种以及两种小鼠物种（小家鼠和西班牙小鼠）之间的限制性片段长度多态性，来确定编码钠钾 - ATP酶（Na +，K + - 激活的ATP磷酸水解酶，EC 3.6.1.3）α和β亚基的基因的染色体定位。针对钠钾 - ATP酶α亚基三种不同同工型的DNA探针分别定位于三条不同的小鼠染色体上：α1基因（Atpa - 1）与3号染色体上的表皮生长因子（Egf）基因共分离；α2（Atpa - 2）与7号染色体上的细胞色素P - 450PB基因家族/香豆素羟化酶基因座共分离；α3（Atpa - 3）与1号染色体上的α - 血影蛋白基因共分离。钠钾 - ATP酶β亚基基因（Atpb）定位于1号染色体的同一区域，但它与Atpa - 3基因并非紧密连锁。这些结果表明，钠钾 - ATP酶α亚基的三种同工型由三个不同的基因编码。钠钾 - ATP酶基因的分散分布表明，它们的表达不太可能受一个共同的顺式作用调控元件控制。
