Álvarez-Manzaneda I, de Vicente I
Departamento de Ecología, Facultad de Ciencias, Universidad de Granada, 18071, Spain; Instituto del Agua, Universidad de Granada, 18071, Spain.
Departamento de Ecología, Facultad de Ciencias, Universidad de Granada, 18071, Spain; Instituto del Agua, Universidad de Granada, 18071, Spain.
Chemosphere. 2017 Nov;187:347-356. doi: 10.1016/j.chemosphere.2017.08.129. Epub 2017 Aug 25.
Laboratory tests, by following standardized Organization for Economic Co-operation and Development (OECD) protocols, were run for evaluating the acute effects of iron magnetic microparticles (MPs), recently proposed for lake restoration, on Chlorella sp. (algal growth) and on the rotifer B. calyciflorus (mortality). In addition, the MPs potential indirect effects on rotifer egg bank were assessed by performing hatching rate test with B. calyciflorus cysts in contact with dissolved iron (Tot-Fe). In the algal growth test, no inhibition occurred at the two lowest MPs concentrations (0.01 and 0.05 g l) which would correspond, considering the adsorption efficiency ratio (Phosphorus: MPs), to P concentrations lower than 0.94 mg P l, much higher than typical concentrations found in natural waters. For higher MPs dose (EC for Chlorella sp. was 0.15 g l), no nutrient limitations but high turbidity and Tot-Fe values cause negative effects on algal growth. For the case of B. calyciflorus, LC was 1.63 g MPs l (corresponding to 30.7 mg P l). When analyzing Tot-Fe effect, the hatching rate of B. calyciflorus cysts was 100% for all treatments. To sum up our results for B. calyciflorus acute and chronic toxicity tests, it is extremely unlikely the mortality of adult organisms in contact with MPs as well as an affectation of the rotifer egg bank. In conclusion, it is expected that MPs addition in a real whole-lake application cause minor lethal and sublethal effects on both Chlorella sp. and B. calyciflorus.
按照经济合作与发展组织（OECD）的标准化协议进行了实验室测试，以评估近期提议用于湖泊修复的铁磁性微颗粒（MPs）对小球藻（藻类生长）和萼花臂尾轮虫（死亡率）的急性影响。此外，通过对与溶解铁（总铁）接触的萼花臂尾轮虫囊肿进行孵化率测试，评估了MPs对轮虫卵库的潜在间接影响。在藻类生长测试中，在两个最低的MPs浓度（0.01和0.05 g/l）下未出现抑制现象，考虑到吸附效率比（磷：MPs），这两个浓度对应的磷浓度低于0.94 mg P/l，远高于天然水体中的典型浓度。对于更高的MPs剂量（小球藻的EC为0.15 g/l），不存在营养限制，但高浊度和总铁值对藻类生长产生负面影响。对于萼花臂尾轮虫，LC为1.63 g MPs/l（相当于30.7 mg P/l）。在分析总铁的影响时，所有处理下萼花臂尾轮虫囊肿的孵化率均为100%。总结我们对萼花臂尾轮虫急性和慢性毒性测试的结果，成年生物接触MPs时极不可能死亡，轮虫卵库也不太可能受到影响。总之，预计在实际的全湖应用中添加MPs对小球藻和萼花臂尾轮虫只会产生轻微的致死和亚致死效应。
