Shepherd Lara D
Museum of New Zealand Te Papa Tongarewa, Wellington, New Zealand.
PLoS One. 2017 Aug 31;12(8):e0183555. doi: 10.1371/journal.pone.0183555. eCollection 2017.
Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for extracting DNA from herbarium specimens. DNA was successfully retrieved from robust leaves and/or stems of herbarium specimens up to 73 years old.
植物标本是植物研究中DNA的重要来源，但目前的采样方法需要去除材料以进行DNA提取。对于珍稀物种或模式标本等不可替代的标本来说，这是不可取的。在此，我提出了第一种从植物标本中提取DNA的非破坏性采样方法。已成功从保存长达73年的植物标本的坚韧叶片和/或茎中提取出DNA。
