Krishna G, Nath J, Petersen M, Ong T
Teratog Carcinog Mutagen. 1987;7(2):183-95. doi: 10.1002/tcm.1770070209.
Sister chromatid exchange (SCE) and chromosomal aberration studies have been used to monitor human populations for genotoxic exposure to chemical substances. These monitoring techniques involve collection of blood and/or bone marrow from the exposed subjects and culturing cells for one or two cell cycles with various treatments in culture. The results obtained from such in vivo/in vitro studies may lead to an over- or underestimation of the damage that could occur in vivo. In the present study, which uses a mouse model, the in vivo/in vitro cytogenetic assays (SCEs and chromosomal aberrations) have been compared with similar in vivo systems in bone marrow and spleen cells treated with various doses of cyclophosphamide (CPA). The results indicate a significant difference in CPA-induced cytogenetic endpoints between in vivo and in vivo/in vitro conditions in both organs. However, linear relationships were found between CPA dose and cytogenetic end point analyzed under both conditions. Based on these results it appears that the in vivo/in vitro assay is a useful technique for indicating potential in vivo damage of chemicals.
姐妹染色单体交换（SCE）和染色体畸变研究已被用于监测人群接触化学物质的遗传毒性。这些监测技术包括从受暴露个体采集血液和/或骨髓，并在培养中用各种处理方法将细胞培养一或两个细胞周期。从这种体内/体外研究获得的结果可能会高估或低估体内可能发生的损伤。在本研究中，使用小鼠模型，将体内/体外细胞遗传学检测（SCE和染色体畸变）与用不同剂量环磷酰胺（CPA）处理的骨髓和脾细胞中的类似体内系统进行了比较。结果表明，在两个器官中，CPA诱导的细胞遗传学终点在体内和体内/体外条件之间存在显著差异。然而，在两种条件下分析时，发现CPA剂量与细胞遗传学终点之间存在线性关系。基于这些结果，体内/体外检测似乎是一种用于指示化学物质潜在体内损伤的有用技术。
