Molecular Genetics Laboratory, Department of Botany, University of North Bengal, Siliguri 734013, West Bengal, India.
Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri 734013, West Bengal, India; Human Nutrition Program, Department of Human Sciences, Ohio State University, Columbus, OH 43210, USA.
J Ethnopharmacol. 2018 Jan 10;210:275-286. doi: 10.1016/j.jep.2017.08.036. Epub 2017 Aug 30.
Acacia nilotica (L.) Delile is used as a traditional anti-diabetic remedy in Bangladesh, Pakistan, Egypt, Nigeria and is mentioned in Ayurveda as well.
The objective of the study was to evaluate the ethnomedicinal claim of A. nilotica leaf (ANL) extract for its efficiency in ameliorating diabetic complications.
ANL was orally administrated (50 and 200mg/kg) to alloxanized mice (blood glucose > 200mg/dL) for 20d. Parameters of glucose metabolism, hepatotoxicity, hyperlipidemia and nephrotoxicity were measured with emphasis on elevated oxidative stress. ANL was chemically characterized using GC-MS. Further, docking studies were employed to predict molecular interactions.
ANL lowered (65%, P< 0.001) systemic glucose load in diabetic mice, which was otherwise 398% higher than control. ANL lowered (35%) insulin resistance, without any significant effect on insulin sensitivity (P> 0.05). Anti-hyperglycemic properties of ANL was further supported by lowering of HbA1c (34%; P< 0.001) and improved glucose utilization (OGTT). Overall diabetic complications were mitigated as reflected by lowered hepatic (ALT, AST) and renal (creatinine, BUN) injury markers and normalization of dyslipidemia. Elevated systemic oxidative stress was lowered by increased catalase and peroxidase activities in liver, kidney and skeletal muscle, resulting in 32% decrease of serum MDA levels. Apart from high phenolic and flavonoid content, tocopherol, catechol and β-sitosterol, identified in ANL, demonstrated substantial binding affinity with Nrf2 protein (5FNQ) reflecting possible crosstalk with intracellular antioxidant defense pathways.
The present study revealed the potentials of A. nilotica to alleviate diabetes-related systemic complications by limiting oxidative stress which justified the ethnopharmacological antidiabetic claim.
金合欢（L.）在孟加拉国、巴基斯坦、埃及、尼日利亚等地被用作传统的抗糖尿病药物，在阿育吠陀医学中也有提及。
本研究旨在评估金合欢叶（ANL）提取物的民族医学用途，以评估其改善糖尿病并发症的功效。
将 ANL 口服给予（50 和 200mg/kg）给所有oxan 化的小鼠（血糖>200mg/dL）20d。重点测量葡萄糖代谢、肝毒性、高血脂和肾毒性等参数，强调氧化应激升高。使用 GC-MS 对 ANL 进行化学表征。此外，还进行了对接研究以预测分子相互作用。
ANL 降低了（65%，P<0.001）糖尿病小鼠的全身葡萄糖负荷，而对照组则高出 398%。ANL 降低（35%）胰岛素抵抗，对胰岛素敏感性无显著影响（P>0.05）。ANL 的抗高血糖特性还通过降低 HbA1c（34%，P<0.001）和改善葡萄糖利用（OGTT）得到进一步支持。肝（ALT、AST）和肾（肌酐、BUN）损伤标志物的降低以及血脂异常的正常化反映了整体糖尿病并发症的缓解。肝、肾和骨骼肌中过氧化氢酶和过氧化物酶活性的增加降低了系统氧化应激，导致血清 MDA 水平降低 32%。除了高酚类和类黄酮含量外，ANL 中鉴定出的生育酚、儿茶酚和β-谷甾醇与 Nrf2 蛋白（5FNQ）具有显著的结合亲和力，反映了与细胞内抗氧化防御途径的可能相互作用。
本研究揭示了金合欢缓解与糖尿病相关的全身并发症的潜力，通过限制氧化应激来缓解糖尿病，这证明了其民族药理学的抗糖尿病功效。
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