Xiao Yang, Wu Xunyao, Ma Lijing, Gui Jingang, Bai Lina, Ni Xin, Wang Jun
Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, Beijing, China; Laboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Immunol Lett. 2017 Nov;191:31-34. doi: 10.1016/j.imlet.2017.08.026. Epub 2017 Aug 30.
Defects in the adaptive immune response to human papillomavirus-6 and -11 are among the most important mechanisms for Juvenile-onset Recurrent Respiratory Papillomatosis (JORRP) development. However, the percentage of CD8+ T cells and peripheral TH1/TH2 immune responses in Juvenile-onset Recurrent Respiratory Papillomatosis is still not well addressed due to limited sample sizes.
Twenty-three patients who were diagnosed with JORRP and underwent surgical intervention at the Beijing TongRen Hospital from October 2015 to March 2016 were enrolled in our study. The CD8 T cells and CD4 T cells in periphery blood were measured by flow cytometry. Intracellular staining was also performed to determine IFN-γ and IL-4 secretion by CD8 T cells and CD4 T cells. Serum IFN-γ and IL-4 levels were measured by ELISA.
We found that the proportions of CD4 and CD8 T cells in peripheral blood of JORRP patients were comparable to that of healthy controls. Moreover, after PMA stimulation, there was no significant change in IFN-γ secretion by either CD4 or CD8 T cells. The secretion of IL-4 but not IFN-γ by CD4 T cells was increased, and the serum IL-4 levels were elevated in JORRP patients.
We conclude that only T2 responses were enhanced but that the T1 responses did not change in the peripheral immunity of JORRP patients.
对人乳头瘤病毒6型和11型适应性免疫反应的缺陷是青少年复发性呼吸道乳头瘤病（JORRP）发病的最重要机制之一。然而，由于样本量有限，青少年复发性呼吸道乳头瘤病中CD8 + T细胞的百分比和外周TH1/TH2免疫反应仍未得到很好的研究。
本研究纳入了2015年10月至2016年3月在北京同仁医院被诊断为JORRP并接受手术干预的23例患者。通过流式细胞术检测外周血中的CD8 T细胞和CD4 T细胞。还进行了细胞内染色以确定CD8 T细胞和CD4 T细胞分泌的IFN-γ和IL-4。通过ELISA检测血清IFN-γ和IL-4水平。
我们发现JORRP患者外周血中CD4和CD8 T细胞的比例与健康对照相当。此外，在PMA刺激后，CD4或CD8 T细胞分泌的IFN-γ没有显著变化。CD4 T细胞分泌的IL-4而非IFN-γ增加，JORRP患者的血清IL-4水平升高。
我们得出结论，在JORRP患者的外周免疫中，仅T2反应增强，而T1反应没有变化。
