Harrison P L, Harrison T, Stockley I, Smith T J
Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK.
Northern General Hospital, Herries Road, Sheffield S5 7AU, UK.
Bone Joint J. 2017 Sep;99-B(9):1153-1156. doi: 10.1302/0301-620X.99B9.BJJ-2016-1309.R1.
Tantalum (Ta) trabecular metal components are increasingly used to reconstruct major bone defects in revision arthroplasty surgery. It is known that some metals such as silver have antibacterial properties. Recent reports have raised the question regarding whether Ta components are protective against infection in revision surgery. This laboratory study aimed to establish whether Ta has intrinsic antibacterial properties against planktonic bacteria, or the ability to inhibit biofilm formation.
Equal-sized pieces of Ta and titanium (Ti) acetabular components were sterilised and incubated with a low dose inoculum of either or for 24 hours. After serial dilution, colony forming units (cfu) were quantified on Mueller-Hinton agar plates. In order to establish whether biofilms formed to a greater extent on one material than the other, these Ta and Ti pieces were then washed twice, sonicated and washed again to remove loosely adhered planktonic bacteria. They were then re-incubated for 24 hours prior to quantifying the number of cfu. All experiments were performed in triplicate.
More than 1x10 cfu/ml were observed in both the Ta and Ti experiments. After washing and sonication, more than 2x10 cfu/ml were observed for both Ta and Ti groups. The results were the same for both and .
Compared with Ti controls, Ta did not demonstrate any intrinsic antibacterial activity or ability to inhibit biofilm formation. Hence, intrinsic antimicrobial properties of Ta do not account for the previously observed reduction in the frequency of subsequent infections when Ta was used in revision procedures. Cite this article: 2017;99-B:1153-6.
钽（Ta）小梁金属部件越来越多地用于翻修关节成形术手术中重建主要骨缺损。已知一些金属如银具有抗菌特性。最近的报道提出了关于Ta部件在翻修手术中是否能预防感染的问题。本实验室研究旨在确定Ta对浮游细菌是否具有内在抗菌特性，或是否具有抑制生物膜形成的能力。
将等尺寸的Ta和钛（Ti）髋臼部件灭菌，并与低剂量接种物（分别为[具体细菌名称1]或[具体细菌名称2]）一起孵育24小时。经过系列稀释后，在穆勒-欣顿琼脂平板上对菌落形成单位（cfu）进行定量。为了确定生物膜在一种材料上的形成程度是否比另一种材料更大，然后将这些Ta和Ti部件洗涤两次，超声处理并再次洗涤以去除松散附着的浮游细菌。然后将它们重新孵育24小时，再对cfu数量进行定量。所有实验均重复进行三次。
在Ta和Ti实验中均观察到超过1×10[具体数值] cfu/ml。洗涤和超声处理后，Ta和Ti组均观察到超过2×10[具体数值] cfu/ml。对于[具体细菌名称1]和[具体细菌名称2]，结果均相同。
与Ti对照相比，Ta未表现出任何内在抗菌活性或抑制生物膜形成的能力。因此，Ta的内在抗菌特性并不能解释在翻修手术中使用Ta时先前观察到的后续感染频率降低的现象。引用本文：[期刊名称] 2017；99-B：1153 - 6。
