Rocha-Penha L, Bettiol H, Barbieri M A, Cardoso V C, Cavalli R C, Sandrim V C
Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Distrito Rubiao Junior, Botucatu, São Paulo, 18680-000, Brazil.
Department of Pediatrics, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, 14049-900, Brazil.
Sci Rep. 2017 Aug 31;7(1):10257. doi: 10.1038/s41598-017-09272-4.
Myeloperoxidase is a proinflammatory enzyme found to be increased in patients with established preeclampsia but never investigated before the disease onset. Here we examined myeloperoxidase concentration and activity in plasma and urine samples from pregnant women who remained normotensive throughout pregnancy and those who developed preeclampsia in order to assess its potential to predict this disorder. Our sample consisted of 30 cases who developed preeclampsia (14 severe and 16 mild) and 57 controls who remained healthy throughout pregnancy, derived from the Brazilian Ribeirão Preto and São Luís prenatal cohort (BRISA). Myeloperoxidase concentration were assessed using a commercial ELISA kit and enzymatic activity through tetramethylbenzidine oxidation. No statistical differences were found in myeloperoxidase levels nor activity between plasma or urine samples from controls, severe and mild cases. Myeloperoxidase did not seem to have a potential application for preeclampsia prediction.
髓过氧化物酶是一种促炎酶，在已患先兆子痫的患者中发现其水平升高，但在疾病发作前从未进行过研究。在此，我们检测了整个孕期血压正常的孕妇以及患先兆子痫孕妇的血浆和尿液样本中的髓过氧化物酶浓度和活性，以评估其预测这种疾病的潜力。我们的样本包括30例患先兆子痫的病例（14例重度和16例轻度）以及57例整个孕期保持健康的对照，这些样本来自巴西里贝朗普雷图和圣路易斯产前队列（BRISA）。使用商用ELISA试剂盒评估髓过氧化物酶浓度，并通过四甲基联苯胺氧化评估酶活性。在对照组、重度和轻度病例的血浆或尿液样本之间，髓过氧化物酶水平和活性均未发现统计学差异。髓过氧化物酶似乎没有用于预测先兆子痫的潜在用途。
