Nim Yap-Shing, Sun Shuangli, Wong Kam-Bo
Centre for Protein Science and Crystallography, Partner State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Methods Mol Biol. 2017;1662:59-73. doi: 10.1007/978-1-4939-7262-3_5.
Homology modeling allows the prediction of a protein structure based on sequence similarity to a known structure of homologous proteins. In this chapter, we use a plant-specific AtSar1a-Atsec23a pair of proteins as a case study to illustrate how to use homology modeling to understand the specificity of the pairwise interaction between AtSar1a and AtSec23a. The detailed procedures described here are also useful in structure prediction of other protein complexes.
同源建模允许基于与同源蛋白质已知结构的序列相似性来预测蛋白质结构。在本章中，我们以植物特有的AtSar1a-Atsec23a这对蛋白质为例，来说明如何使用同源建模来理解AtSar1a和AtSec23a之间成对相互作用的特异性。这里描述的详细程序在其他蛋白质复合物的结构预测中也很有用。
