García-Arrarás J E, Chanconie M, Ziller C, Fauquet M
Brain Res. 1987 Jun;430(2):255-65. doi: 10.1016/0165-3806(87)90158-1.
Qualitative and quantitative in vivo studies were performed on the development of the neuropeptide vasoactive intestinal polypeptide (VIP) in the peripheral nervous system of quail embryos. VIP-like immunoreactivity (VIPLI) was found by radioimmunoassay (RIA) from the sixth day of embryonic life onward in the sympathetic chain, the esophagus and duodenum, and from day 15 of incubation onward in the adrenal glands and the nodose ganglia. By using immunocytochemistry, we identified cells expressing VIPLI in sensory spinal ganglia of 13- to 15-day-old embryos. In neural crest cultures, cells expressing the VIP phenotype differentiated constantly under various culture conditions, in contrast to other phenotypes which had specific medium requirements, i.e. adrenergic cells or substance P-containing neurons.
对鹌鹑胚胎外周神经系统中神经肽血管活性肠多肽（VIP）的发育进行了定性和定量的体内研究。通过放射免疫分析（RIA）发现，从胚胎生命的第六天起，在交感神经链、食管和十二指肠中可检测到VIP样免疫反应性（VIPLI），从孵化第15天起，在肾上腺和结状神经节中也可检测到。通过免疫细胞化学方法，我们在13至15日龄胚胎的感觉脊髓神经节中鉴定出表达VIPLI的细胞。在神经嵴培养物中，与其他有特定培养基要求的表型（即肾上腺素能细胞或含P物质的神经元）不同，表达VIP表型的细胞在各种培养条件下持续分化。
