Hayashi M, Edgar D, Thoenen H
Neuroscience. 1983 Sep;10(1):31-9. doi: 10.1016/0306-4522(83)90078-7.
Antisera to substance P, somatostatin and vasoactive intestinal polypeptide were produced in rabbits and used to establish radioimmunoassays. The specificities and sensitivities of the assays were characterised before using them to measure the levels of these peptides in the paravertebral sympathetic ganglia and spinal sensory ganglia of embryonic chicks. The immunoreactive materials in the developing ganglia were shown to be identical with the peptides by the criterion of co-elution on reverse-phase high performance liquid chromatography. The levels of the peptides varied independently during the developmental period studied between day 8 and hatching at day 20 of incubation. Vasoactive intestinal polypeptide was first detectable on day 12 in the sympathetic ganglia and the levels increased through to hatching, at which time it first became detectable in the sensory ganglia. In contrast, substance P and somatostatin were detectable in both sensory and sympathetic ganglia throughout the period studied: the content of substance P increased up to day 18 after which time it declined, and somatostatin was present initially (day 8) at high levels before rapidly declining to a minimum at day 10 and then slowly increasing again, in parallel with the general growth of the ganglia, reflected by their protein contents. The differential changes in levels of substance P, somatostatin and vasoactive intestinal polypeptide during development indicates that different regulatory mechanisms are responsible for the ontogenesis of individual peptide phenotypes in the peripheral nervous system.
针对P物质、生长抑素和血管活性肠肽的抗血清在兔体内产生，并用于建立放射免疫测定法。在使用这些测定法测量胚胎小鸡椎旁交感神经节和脊髓感觉神经节中这些肽的水平之前，对其特异性和敏感性进行了表征。通过反相高效液相色谱上的共洗脱标准，证明发育中的神经节中的免疫反应性物质与这些肽相同。在研究的发育期间（从孵化第8天到第20天孵化），这些肽的水平独立变化。血管活性肠肽在交感神经节中于第12天首次可检测到，其水平一直增加到孵化时，此时它在感觉神经节中首次变得可检测到。相比之下，在整个研究期间，P物质和生长抑素在感觉神经节和交感神经节中均可检测到：P物质的含量在第18天之前增加，之后下降，生长抑素最初（第8天）含量很高，然后在第10天迅速下降到最低水平，然后再次缓慢增加，与神经节的总体生长平行，神经节的总体生长由其蛋白质含量反映。发育过程中P物质、生长抑素和血管活性肠肽水平的差异变化表明，不同的调节机制负责外周神经系统中单个肽表型的个体发生。
