Zheng Yong-Feng, Wang Lei, Zhao Si-Lei, Gan Yan-Xiong, Yu Jia-Li, Liao Wan, Fu Chao-Mei
Pharmacy School of Chengdu University of Traditional Chinese Medicine State Key Lab Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources-Co-Founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jan;41(2):257-263. doi: 10.4268/cjcmm20160216.
To compare the differences of essential oils extracted from Curcumae Rhizoma with different origins. The TIC of the essential oils of Curcumae Rhizoma from three different origins recorded by CP(2010) , were investigated by GC-MS combined with automated mass spectral deconvolution and identification system(AMDIS),steps as follow: firstly, overlapped peaks were resolved by AMDIS,secondly, NIST11.L standard MS spectral database combined with retention index were used to assist qualitative analysis, thirdly, the peak area of each split peak were determined by choosing the characteristic fragment ion peak, finally, the relative percentage contents of each compounds were determined through peak area normalization method. The results showed that the components of the essential oils of Curcumae Rhizoma from three origins were different from each other at various degree. In conclusion, the types and content of essential oils could be an indicator to evaluate the quality of Curcumae Rhizoma from different origins and its geoherbalism.
比较不同产地莪术挥发油的差异。采用气相色谱 - 质谱联用技术结合自动质谱解卷积与鉴定系统(AMDIS),对《中国药典》(2010年版)收录的3个不同产地莪术挥发油的总离子流图进行研究,步骤如下:首先,利用AMDIS解析重叠峰;其次,采用NIST11.L标准质谱数据库结合保留指数辅助定性分析;再次,选择特征碎片离子峰确定各分离峰的峰面积;最后,通过峰面积归一化法确定各化合物的相对百分含量。结果表明,3个产地莪术挥发油的成分在不同程度上存在差异。综上所述,挥发油的种类和含量可作为评价不同产地莪术质量及其道地性的指标。