GC-MS 法测定大鼠口服莪术油后血浆中 7 种挥发性成分及其药代动力学研究。
GC-MS method for determination and pharmacokinetic study of seven volatile constituents in rat plasma after oral administration of the essential oil of Rhizoma Curcumae.
机构信息
College of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China; College of Pharmacy, Qiqihar Medical University, 333 Buikui Street, Qiqihar, 161006, PR China.
College of Pharmacy, Qiqihar Medical University, 333 Buikui Street, Qiqihar, 161006, PR China.
出版信息
J Pharm Biomed Anal. 2018 Feb 5;149:577-585. doi: 10.1016/j.jpba.2017.11.058. Epub 2017 Nov 28.
Rhizoma Curcumae (RC) is perennial herbaceous plant mainly present in China, India and Malaysiabelong, which is belong to the family Zingiberaceae. The rhizomes of RC have been used as a famous traditional Chinese medicine for the treatment of syndrome of blood stasis. A selective, sensitive and accurate gas chromatography-mass spectroscopy (GC-MS) method was developed and validated in this paper for the simultaneous determination and pharmacokinetic study of α-Pinene, 1,8-Cineole, Borneol, β-Elemene, Curcumol, Germacrone, and Curdione in rat plasma. The GC-MS system was operated under selected ion monitoring (SIM) mode using a DB-5 (30m×0.25mm (ID)×0.25μm (film thickness)) column. Linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability were used to validate the current GC/MS assay. The lowest limit of quantifications (LLOQ) of α-Pinene, 1,8-Cineole, Borneol, β-Elemene, Curcumol, Germacrone, Curdione were 2.71ng/mL, 7.76ng/mL, 3.37ng/mL, 21.68ng/mL, 40.21ng/mL, 24.84ng/mL and 47.78ng/mL respectively. After oral administration 1.0g/kg of RC rhizomes to the rats, the maximum plasma concentration (C) was 34.72±9.97ng/mL for α-Pinene, 99.86±5.54ng/mL for 1,8-Cineole, 16.10±3.37ng/mL for Borneol, 248.98±86.19ng/mL for β-Elemene, 673.75±104.15ng/mL for Curcumol, 2353.64±637.83ng/mL for Germacrone and 2420.04±708.51ng/mL for Curdione. The time to reach the maximum plasma concentration (T) was 2.33±0.29h for α-Pinene, 0.67±0.29h for 1,8-Cineole, 1.33±0.58h for Borneol, 1.83±0.76h for β-Elemene, 0.83±0.29h for Curcumol, 0.89±0.98h for Germacrone and 1.17±0.76h for Curdione. In this study, a validated GC-MS method for simultaneous determination of seven volatile oil compounds in rat plasma after oral administration of the extract of RC rhizomes and research on their pharmacokinetics was validated. The recovery and stability results were satisfactory in this study.
莪术根茎（RC）是一种多年生草本植物，主要分布在中国、印度和马来西亚，属于姜科。RC 的根茎已被用作一种著名的中药，用于治疗血瘀综合征。本文建立并验证了一种选择性、灵敏性和准确性的气相色谱-质谱（GC-MS）方法，用于同时测定和研究大鼠血浆中α-蒎烯、1,8-桉叶素、龙脑、β-榄香烯、莪术醇、大根香叶烯 D 和莪术二酮的药代动力学。GC-MS 系统采用 DB-5（30m×0.25mm（ID）×0.25μm（膜厚））柱，在选择离子监测（SIM）模式下运行。使用最低检测限（LLOQ）、日内和日间精密度、准确度、提取回收率和稳定性来验证当前的 GC/MS 分析方法。α-蒎烯、1,8-桉叶素、龙脑、β-榄香烯、莪术醇、大根香叶烯 D 和莪术二酮的最低检测限分别为 2.71ng/mL、7.76ng/mL、3.37ng/mL、21.68ng/mL、40.21ng/mL、24.84ng/mL 和 47.78ng/mL。大鼠口服 1.0g/kg RC 根茎提取物后，α-蒎烯的最大血浆浓度（C）为 34.72±9.97ng/mL，1,8-桉叶素为 99.86±5.54ng/mL，龙脑为 16.10±3.37ng/mL，β-榄香烯为 248.98±86.19ng/mL，莪术醇为 673.75±104.15ng/mL，大根香叶烯 D 为 2353.64±637.83ng/mL，莪术二酮为 2420.04±708.51ng/mL。达到最大血浆浓度（T）的时间为：α-蒎烯为 2.33±0.29h，1,8-桉叶素为 0.67±0.29h，龙脑为 1.33±0.58h，β-榄香烯为 1.83±0.76h，莪术醇为 0.83±0.29h，大根香叶烯 D 为 0.89±0.98h，莪术二酮为 1.17±0.76h。本研究建立了一种同时测定莪术根茎提取物灌胃后大鼠血浆中七种挥发性油类化合物的 GC-MS 方法，并对其药代动力学进行了研究。本研究中的回收率和稳定性结果令人满意。
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