Brudar Sandi, Černigoj Urh, Podgornik Helena, Kržan Mojca, Prislan Iztok
Acta Chim Slov. 2017 Sep;64(3):564-570. doi: 10.17344/acsi.2016.2970.
Differential scanning calorimetry provides unique signatures of blood plasma samples. Plasma samples from diseased individuals yield specific thermograms, which differ from each other and from plasma samples of healthy individuals. Thermograms from individuals suffering from chronic lymphocytic leukemia, multiple myeloma and acute myeloid leukemia were measured with DSC. To obtain additional information about thermal behaviour of plasma proteins immunoaffinity chromatography was introduced. An immunoextraction of HSA using a chromatographic column with immobilized anti-HSA was carried out in order to enrich less abundant plasma proteins, which could provide a further insight into disease development. Efficiency of HSA depletion and protein composition of fractionated plasma was validated by SDS-PAGE.
差示扫描量热法可提供血浆样本的独特特征。患病个体的血浆样本会产生特定的热谱图，这些热谱图彼此不同，且与健康个体的血浆样本也不同。使用差示扫描量热法测量了慢性淋巴细胞白血病、多发性骨髓瘤和急性髓细胞白血病患者的热谱图。为了获得有关血浆蛋白热行为的更多信息，引入了免疫亲和色谱法。使用固定化抗人血清白蛋白的色谱柱对人血清白蛋白进行免疫提取，以富集含量较少的血浆蛋白，这有助于进一步了解疾病发展情况。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳验证了人血清白蛋白去除效率和分级分离血浆的蛋白质组成。
